Using multidimensional projection techniques for reaching a high distinguishing ability in biosensing

Using multidimensional projection techniques for reaching a high distinguishing ability in biosensing

Author Paulovich, Fernando V. Google Scholar
Maki, Rafael M. Google Scholar
Oliveira, Maria C. F. de Google Scholar
Colhone, Marcelle C. Google Scholar
Santos, Fabiana R. Google Scholar
Migliaccio, Vanessa Google Scholar
Ciancaglini, Pietro Google Scholar
Perez, Katia R. Autor UNIFESP Google Scholar
Stabeli, Rodrigo G. Google Scholar
Perinoto, Angelo C. Google Scholar
Oliveira, Osvaldo N. Google Scholar
Zucolotto, Valtencir Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rondonia UNIR
Abstract Recent advances in the control of molecular engineering architectures have allowed unprecedented ability of molecular recognition in biosensing, with a promising impact for clinical diagnosis and environment control. the availability of large amounts of data from electrical, optical, or electrochemical measurements requires, however, sophisticated data treatment in order to optimize sensing performance. in this study, we show how an information visualization system based on projections, referred to as Projection Explorer (PEx), can be used to achieve high performance for biosensors made with nanostructured films containing immobilized antigens. As a proof of concept, various visualizations were obtained with impedance spectroscopy data from an array of sensors whose electrical response could be specific toward a given antibody (analyte) owing to molecular recognition processes. in addition to discussing the distinct methods for projection and normalization of the data, we demonstrate that an excellent distinction can be made between real samples tested positive for Chagas disease and Leishmaniasis, which could not be achieved with conventional statistical methods. Such high performance probably arose from the possibility of treating the data in the whole frequency range. Through a systematic analysis, it was inferred that Sammon's mapping with standardization to normalize the data gives the best results, where distinction could be made of blood serum samples containing 10(-7) mg/mL of the antibody. the method inherent in PEx and the procedures for analyzing the impedance data are entirely generic and can be extended to optimize any type of sensor or biosensor.
Keywords Biosensors
Nanostructured films
Leishmaniasis
Chagas disease
Projection techniques
Information visualization
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
GENOPROT
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2011-05-01
Published in Analytical and Bioanalytical Chemistry. Heidelberg: Springer Heidelberg, v. 400, n. 4, p. 1153-1159, 2011.
ISSN 1618-2642 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1153-1159
Origin http://dx.doi.org/10.1007/s00216-011-4853-2
Access rights Closed access
Type Article
Web of Science ID WOS:000289432500023
URI http://repositorio.unifesp.br/handle/11600/33663

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