Cysteine Cathepsins in Human Carious Dentin

Cysteine Cathepsins in Human Carious Dentin

Author Nascimento, F. D. Google Scholar
Minciotti, C. L. Google Scholar
Geraldeli, S. Google Scholar
Carrilho, M. R. Google Scholar
Pashley, D. H. Google Scholar
Tay, F. R. Google Scholar
Nader, H. B. Autor UNIFESP Google Scholar
Salo, T. Google Scholar
Tjaderhane, L. Google Scholar
Tersariol, I. L. S. Autor UNIFESP Google Scholar
Institution Univ Mogi das Cruzes
Univ Bandeirante São Paulo UNIBAN
Univ Iowa
Med Coll Georgia
Universidade Federal de São Paulo (UNIFESP)
Univ Oulu
Oulu Univ Hosp
Abstract Matrix metalloproteinases (MMPs) are important in dentinal caries, and analysis of recent data demonstrates the presence of other collagen-degrading enzymes, cysteine cathepsins, in human dentin. This study aimed to examine the presence, source, and activity of cysteine cathepsins in human caries. Cathepsin B was detected with immunostaining. Saliva and dentin cysteine cathepsin and MMP activities on caries lesions were analyzed spectrofluorometrically. Immunostaining demonstrated stronger cathepsins B in carious than in healthy dentin. in carious dentin, cysteine cathepsin activity increased with increasing depth and age in chronic lesions, but decreased with age in active lesions. MMP activity decreased with age in both active and chronic lesions. Salivary MMP activities were higher in patients with active than chronic lesions and with increasing lesion depth, while cysteine cathepsin activities showed no differences. the results indicate that, along with MMPs, cysteine cathepsins are important, especially in active and deep caries.
Keywords MMP
enzyme
cathepsin
human
tooth
dentin
Language English
Sponsor Academy of Finland
Finnish Dental Society Apollonia
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
National Institute of Dental and Craniofacial Research
Grant number National Institute of Dental and Craniofacial Research: R01 DE015306-06
National Institute of Dental and Craniofacial Research: R21 DE019213-02
Date 2011-04-01
Published in Journal of Dental Research. Thousand Oaks: Sage Publications Inc, v. 90, n. 4, p. 506-511, 2011.
ISSN 0022-0345 (Sherpa/Romeo, impact factor)
Publisher Sage Publications Inc
Extent 506-511
Origin http://dx.doi.org/10.1177/0022034510391906
Access rights Closed access
Type Article
Web of Science ID WOS:000288798600018
URI http://repositorio.unifesp.br/handle/11600/33622

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