Enhancement of blood porphyrin emission intensity with aminolevulinic acid administration: A new concept for photodynamic diagnosis of early prostate cancer

Enhancement of blood porphyrin emission intensity with aminolevulinic acid administration: A new concept for photodynamic diagnosis of early prostate cancer

Author Silva, Flávia Rodrigues de Oliveira Autor UNIFESP Google Scholar
Bellini, Maria Helena Autor UNIFESP Google Scholar
Nabeshima, Camila Tiemi Autor UNIFESP Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Vieira, Nilson Dias Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Ctr Lasers & Aplicacoes
Abstract Background: the objective of this paper was to verify if the oral administration of delta-aminolevulinic acid (ALA) in animals with prostate tumor can increase the sensitivity of cancer diagnosis by protoporphyrin IX blood autofluorescence. in this study, the autofluorescence of blood porphyrin was analyzed using fluorescence spectroscopy on healthy male NUDE mice and in those with prostate cancer induced by the inoculation of DU145 cells.Methods: A total of 18 male NUDE mice, similar to 8 weeks old on arrival were divided into 3 groups: Control, Tumor and ALA Tumor. the autofluorescence of blood porphyrin of the groups was analyzed using fluorescence spectroscopy at different days after tumor induction, to monitor the tumor progression. Emission spectra were obtained by exciting the samples at 405 nm. the animals inoculated had their blood collected with and without oral ALA solution administration to compare PPIX endogenous (Tumor group) and exogenous (ALA Tumor group) signal intensity and to establish a method to diagnosis early prostate cancer.Results: Significant differences were observed in autofluorescence intensities measured in the 575-725 nm spectral regions for the studied groups.Conclusions: the results showed an enhancement of almost 100% in blood PPIX fluorescence, using the oral administration of delta-aminolevulinic acid on male NUDE mice with prostate cancer, making fluorescence measurements more accurate and sensitive since the first week after tumor induction. (C) 2010 Elsevier B.V. All rights reserved.
Keywords Aminolevulinic acid
Porphyrin
Tumor stage
Prostate cancer
Photodynamic diagnosis
Language English
Date 2011-03-01
Published in Photodiagnosis and Photodynamic Therapy. Amsterdam: Elsevier B.V., v. 8, n. 1, p. 7-13, 2011.
ISSN 1572-1000 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 7-13
Origin http://dx.doi.org/10.1016/j.pdpdt.2010.12.006
Access rights Closed access
Type Article
Web of Science ID WOS:000288525100002
URI http://repositorio.unifesp.br/handle/11600/33533

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