Kallikrein 1 is overexpressed by astrocytes in the hippocampus of patients with refractory temporal lobe epilepsy, associated with hippocampal sclerosis

Kallikrein 1 is overexpressed by astrocytes in the hippocampus of patients with refractory temporal lobe epilepsy, associated with hippocampal sclerosis

Author Rodrigues Simoes, Priscila Santos Autor UNIFESP Google Scholar
Perosa, Sandra Regina Autor UNIFESP Google Scholar
Arganaraz, Gustavo Adolfo Google Scholar
Yacubian, Elza Márcia Targas Autor UNIFESP Google Scholar
Carrete, Henrique Autor UNIFESP Google Scholar
Centeno, Ricardo Silva Autor UNIFESP Google Scholar
Vasconcellos Varella, Pedro Paulo Autor UNIFESP Google Scholar
Carvalho Santiago, Joselita Ferreira Autor UNIFESP Google Scholar
Canzian, Mauro Google Scholar
Silva, Jose Antonio Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Amado, Debora Autor UNIFESP Google Scholar
Cavalheiro, Esper Abrao Autor UNIFESP Google Scholar
Naffah Mazzacoratti, Maria da Graca Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande do Norte
Universidade de São Paulo (USP)
Univ Nove de Julho
Abstract Kallikrein 1 (hK1) is a tissue enzyme responsible for kinin release in inflammatory cascade. This study was delineated to study the distribution and the co-localization of hK1 and kinin B1 and B2 receptors with glial and/or neuronal proteins markers, in the hippocampus of patients with refractory temporal lobe epilepsy, associated with hippocampal sclerosis (TLE-HS), comparing with control tissues. Hippocampal levels of KLK1 mRNA were also measured.hK1, kinin B1 and B2 receptors, NeuN and GFAP were analyzed using immunohistochemistry and confocal microscopy and KLK1 mRNA was quantified with real time PCR.Increased expression of hK1 by astrocytes co-localized with GFAP was found, contrasting with kinin B1 and B2 receptors, which were co-localized with NeuN in the sclerotic hippocampus. in addition, KLK1 mRNA was also up-regulated in same tissues.These data suggest an overexpression of kallikrein-kinin system and a neuron-glia interaction in the inflammatory process present in refractory TLE-HS. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Temporal lobe epilepsy
Kallikrein 1
Kinin receptors
Astrocytes and neurons
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
CPNq
Instituto Nacional de Neurociencia Translacional (INNT)
Date 2011-03-01
Published in Neurochemistry International. Oxford: Pergamon-Elsevier B.V., v. 58, n. 4, p. 477-482, 2011.
ISSN 0197-0186 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 477-482
Origin http://dx.doi.org/10.1016/j.neuint.2010.12.021
Access rights Closed access
Type Article
Web of Science ID WOS:000288774800005
URI http://repositorio.unifesp.br/handle/11600/33532

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