Adolescent varicocele: improved sperm function after varicocelectomy

Show simple item record

dc.contributor.author Lacerda, Jose Iran [UNIFESP]
dc.contributor.author Del Giudice, Paula Toni [UNIFESP]
dc.contributor.author Silva, Barbara Ferreira da [UNIFESP]
dc.contributor.author Nichi, Marcilio
dc.contributor.author Fariello, Roberta Maria [UNIFESP]
dc.contributor.author Fraietta, Renato [UNIFESP]
dc.contributor.author Restelli, Adriana Ester [UNIFESP]
dc.contributor.author Blumer, Camile Garcia [UNIFESP]
dc.contributor.author Bertolla, Ricardo Pimenta [UNIFESP]
dc.contributor.author Cedenho, Agnaldo Pereira [UNIFESP]
dc.date.accessioned 2016-01-24T14:06:13Z
dc.date.available 2016-01-24T14:06:13Z
dc.date.issued 2011-03-01
dc.identifier http://dx.doi.org/10.1016/j.fertnstert.2010.10.031
dc.identifier.citation Fertility and Sterility. New York: Elsevier B.V., v. 95, n. 3, p. 994-999, 2011.
dc.identifier.issn 0015-0282
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/33482
dc.description.abstract Objective: To assess the effect of varicocelectomy on sperm function (DNA integrity and mitochondrial activity) and levels of lipid peroxidation in seminal plasma of adolescents.Design: Prospective study.Setting: Patients recruited from a local public school.Patient(s): Adolescents (14-19 years old), Tanner stages IV or V with varicocele grades II or III, attending a local public school.Intervention(s): Two semen collections with a one week interval between collections before bilateral varicocele repair using subinguinal microsurgical varicocelectomy, and two semen collections with a one week interval between collections three months after the surgery.Main Outcome Measure(s): Rate of sperm DNA fragmentation as assessed by the Comet assay and categorized as classes I (no DNA fragmentation) to IV (high DNA fragmentation). Rate of mitochondrial activity as assessed by the diaminobenzidine assay and categorized as grades I (all mitochondria active) to IV (all mitochondria inactive). Levels of lipid peroxidation in seminal plasma by a colorimetric method that quantifies a lipid peroxidation subproduct (malondialdehyde).Result(s): Concerning DNA integrity, the samples after varicocelectomy showed more spermatozoa with intact nuclear DNA (grade I) and less spermatozoa with Comet grades II, III, and IV. Regarding mitochondrial activity, the samples after varicocelectomy showed less cells with inactive mitochondria (class III). No differences were observed in classes I, II, and IV. Concerning lipid peroxidation, no significant differences were observed.Conclusion(s): This study was able to demonstrate that varicocelectomy in adolescents is associated with increased sperm DNA integrity and mitochondrial activity. However, levels of seminal products of lipid degradation (malondialdehyde) are not different. (Fertil Steril (R) 2011;95:994-9. (C) 2011 by American Society for Reproductive Medicine.) en
dc.format.extent 994-999
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Fertility and Sterility
dc.rights Acesso aberto
dc.subject Varicocele en
dc.subject DNA damage en
dc.subject mitochondria/metabolism en
dc.subject lipid peroxidation en
dc.subject adolescents en
dc.title Adolescent varicocele: improved sperm function after varicocelectomy en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Universidade Federal de São Paulo, Human Reprod Sect, Div Urol, Dept Surg, BR-03320000 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Anim Reprod, Sch Vet Med, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Human Reprod Sect, Div Urol, Dept Surg, BR-03320000 São Paulo, Brazil
dc.identifier.doi 10.1016/j.fertnstert.2010.10.031
dc.description.source Web of Science
dc.identifier.wos WOS:000287480300029



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account