Adolescent varicocele: improved sperm function after varicocelectomy

Adolescent varicocele: improved sperm function after varicocelectomy

Author Lacerda, Jose Iran Autor UNIFESP Google Scholar
Del Giudice, Paula Toni Autor UNIFESP Google Scholar
Silva, Barbara Ferreira da Autor UNIFESP Google Scholar
Nichi, Marcilio Google Scholar
Fariello, Roberta Maria Autor UNIFESP Google Scholar
Fraietta, Renato Autor UNIFESP Google Scholar
Restelli, Adriana Ester Autor UNIFESP Google Scholar
Blumer, Camile Garcia Autor UNIFESP Google Scholar
Bertolla, Ricardo Pimenta Autor UNIFESP Google Scholar
Cedenho, Agnaldo Pereira Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Objective: To assess the effect of varicocelectomy on sperm function (DNA integrity and mitochondrial activity) and levels of lipid peroxidation in seminal plasma of adolescents.Design: Prospective study.Setting: Patients recruited from a local public school.Patient(s): Adolescents (14-19 years old), Tanner stages IV or V with varicocele grades II or III, attending a local public school.Intervention(s): Two semen collections with a one week interval between collections before bilateral varicocele repair using subinguinal microsurgical varicocelectomy, and two semen collections with a one week interval between collections three months after the surgery.Main Outcome Measure(s): Rate of sperm DNA fragmentation as assessed by the Comet assay and categorized as classes I (no DNA fragmentation) to IV (high DNA fragmentation). Rate of mitochondrial activity as assessed by the diaminobenzidine assay and categorized as grades I (all mitochondria active) to IV (all mitochondria inactive). Levels of lipid peroxidation in seminal plasma by a colorimetric method that quantifies a lipid peroxidation subproduct (malondialdehyde).Result(s): Concerning DNA integrity, the samples after varicocelectomy showed more spermatozoa with intact nuclear DNA (grade I) and less spermatozoa with Comet grades II, III, and IV. Regarding mitochondrial activity, the samples after varicocelectomy showed less cells with inactive mitochondria (class III). No differences were observed in classes I, II, and IV. Concerning lipid peroxidation, no significant differences were observed.Conclusion(s): This study was able to demonstrate that varicocelectomy in adolescents is associated with increased sperm DNA integrity and mitochondrial activity. However, levels of seminal products of lipid degradation (malondialdehyde) are not different. (Fertil Steril (R) 2011;95:994-9. (C) 2011 by American Society for Reproductive Medicine.)
Keywords Varicocele
DNA damage
lipid peroxidation
Language English
Date 2011-03-01
Published in Fertility and Sterility. New York: Elsevier B.V., v. 95, n. 3, p. 994-999, 2011.
ISSN 0015-0282 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 994-999
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000287480300029

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