Observing the Solubilization of Lipid Bilayers by Detergents with Optical Microscopy of GUVs

Observing the Solubilization of Lipid Bilayers by Detergents with Optical Microscopy of GUVs

Author Sudbrack, Tatiane P. Google Scholar
Archilha, Nathaly L. Google Scholar
Itri, Rosangela Google Scholar
Riske, Karin A. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract The solubilization of lipid bilayers by detergents was studied with optical microscopy of giant unilamellar vesicles (GUVs) composed of palmitoyl oleoyl phoshatidylcholine (POPC). A solution of the detergents Triton X-100 (TX-100) and sodium dodecyl sulfate (SDS) was injected with a micropipette close to single GUVs. the solubilization process was observed with phase contrast and fluorescence microscopy and found to be dependent on the detergent nature. in the presence of TX-100, GUVs initially showed an increase in their surface area, due to insertion of TX-100 with rapid equilibration between the two leaflets of the bilayer. Then, above a solubility threshold, several holes opened, rendering the bilayer a lace fabric appearance, and the bilayer gradually vanished. On the other hand, injection of SDS caused initially an increase in the membrane spontaneous curvature, which is mainly associated with incorporation of SDS in the outer layer only. This created a stress in the membrane, which caused either opening of transient macropores with substantial decrease in vesicle size or complete vesicle bursting. in another experimental setup, the extent of solubilization/destruction of a collection of GUVs was measured as a function of either TX-100 or SDS concentration.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
inctFCx
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2011-01-20
Published in Journal of Physical Chemistry B. Washington: Amer Chemical Soc, v. 115, n. 2, p. 269-277, 2011.
ISSN 1520-6106 (Sherpa/Romeo, impact factor)
Publisher Amer Chemical Soc
Extent 269-277
Origin http://dx.doi.org/10.1021/jp108653e
Access rights Closed access
Type Article
Web of Science ID WOS:000286090200007
URI http://repositorio.unifesp.br/handle/11600/33358

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