The A(1) receptor agonist R-Pia reduces the imbalance between cerebral glucose metabolism and blood flow during status epilepticus: Could this mechanism be involved with neuroprotection?

The A(1) receptor agonist R-Pia reduces the imbalance between cerebral glucose metabolism and blood flow during status epilepticus: Could this mechanism be involved with neuroprotection?

Author Silva, Lara Ribeiro Autor UNIFESP Google Scholar
Nehlig, Astrid Google Scholar
Rosim, Fernanda Elisa Autor UNIFESP Google Scholar
Vignoli, Thiago Autor UNIFESP Google Scholar
Persike, Daniele Suzete Autor UNIFESP Google Scholar
Ferrandon, Arielle Google Scholar
Sinigaglia-Coimbra, Rita Autor UNIFESP Google Scholar
Silva Fernandes, Maria Jose da Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Strasbourg
Abstract It is well known that the uncoupling between local cerebral glucose utilization (LCGU) and local cerebral blood flow (LCBF), i.e. decrease in LCBF rates with high LCGU, is frequently associated with seizure-induced neuronal damage. This study was performed to assess if the neuroprotective effect of the adenosinergic A(1) receptor agonist R-N-phenylisopropyladenosine (R-Pia) injected prior to pilocarpine is able to reduce the uncoupling between LCGU and LCBF during status epilepticus (SE). Four groups of rats were studied: Saline, Pilo, R-Pia + Saline and R-Pia + Pilo. for LCGU and LCBF studies, rats were subjected to autoradiography using [C-14]-2-deoxyglucose and [C-14]-iodoantypirine, respectively. Radioligands were injected 4 h after SE onset. Neuronal loss was evaluated by Fluorojade-B (FJB) at two time points after SE onset (24 h and 7 days). the results showed a significant increase in LCGU in almost all brain regions studied in the Pilo and R-Pia + Pilo groups compared to controls. However, in R-Pia pretreated rats, the uncoupling between LCGU and LCBF was moderated in a limited number of structures as substantia nigra pars reticulata and hippocampal formation rather in favor of hyperperfusion. Significant increases in LCBF were observed in the entorhinal cortex, thalamic nuclei, mammillary body, red nucleus, zona incerta, pontine nucleus and visual cortex. the neuroprotective effect of R-Pia assessed by FJB showed a lower density of degenerating cells in the hippocampal formation, piriform cortex and basolateral amygdala. in conclusion our data shows that the neuroprotective effect of R-Pia was accompanied by a compensatory metabolic input in brain areas involved with seizures generation. Published by Elsevier Inc.
Keywords Pilocarpine
Neuroprotection
Adenosine receptor
R-Pia, glucose metabolism
Cerebral blood flow
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2011-01-01
Published in Neurobiology of Disease. San Diego: Academic Press Inc Elsevier Science, v. 41, n. 1, p. 169-176, 2011.
ISSN 0969-9961 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 169-176
Origin http://dx.doi.org/10.1016/j.nbd.2010.09.004
Access rights Closed access
Type Article
Web of Science ID WOS:000285036400018
URI http://repositorio.unifesp.br/handle/11600/33283

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