Dual candidemia detected by nested polymerase chain reaction in two critically ill children

Dual candidemia detected by nested polymerase chain reaction in two critically ill children

Author del Negro, G. M. B. Google Scholar
Delgado, A. F. Google Scholar
Manuli, E. R. Google Scholar
Yamamoto, L. Google Scholar
Okay, T. S. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract The use of improved microbiological procedures associated with molecular techniques has increased the identification of Candida bloodstream infections, even if the isolation of more than one species by culture methods remains uncommon. We report the cases of two children presenting with severe gastrointestinal disorders and other risk factors that contribute to Candida infections. in the first patient, C. albicans DNA was initially detected by a nested-amplification and C. tropicalis was found later during hospitalization, while blood cultures were persistently negative. in the second child, there was amplification of C. albicans and C. glabrata DNA in the same samples, but blood cultures yielded only C. albicans. Both patients received antifungal therapy but had unfavorable outcomes. These two cases illustrate that PCR was more successful than culture methods in detecting Candida in the bloodstream of high risk children, and was also able to detect the presence of more than one species in the same patient that might impact therapy when the fungi are resistant to azole compounds.</.
Keywords Candida spp.
PCR
polyfungemia
pediatric candidiasis
Language English
Date 2010-12-01
Published in Medical Mycology. London: Informa Healthcare, v. 48, n. 8, p. 1116-1120, 2010.
ISSN 1369-3786 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 1116-1120
Origin http://dx.doi.org/10.3109/13693786.2010.499375
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000284169200016
URI http://repositorio.unifesp.br/handle/11600/33104

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