ACE2-angiotensin-(1-7)-Mas axis in renal ischaemia/reperfusion injury in rats

ACE2-angiotensin-(1-7)-Mas axis in renal ischaemia/reperfusion injury in rats

Author Silveira, Katia D. da Google Scholar
Bosco, Kenia S. Pompermayer Google Scholar
Diniz, Lucio R. L. Google Scholar
Carmona, Adriana Karaoglanovic Autor UNIFESP Google Scholar
Cassali, Giovanni D. Google Scholar
Bruna-Romero, Oscar Google Scholar
Sousa, Lirlandia P. de Google Scholar
Teixeira, Mauro M. Google Scholar
Santos, Robson A. S. Google Scholar
Simoes e Silva, Ana C. Google Scholar
Ribeiro Vieira, Maria A. Google Scholar
Institution Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Abstract AngII (angiotensin II), ACE (angiotensin I-converting enzyme) and the AT(1) receptor (AngII type I receptor) are associated with the inflammatory process and microvascular dysfunction of AKI (acute kidney injury) induced by renal I/R (ischaemia/reperfusion). However, Ang-(1-7) [angiotensin-(1-7)], ACE2 (angiotensin I-converting enzyme 2) and the Mas receptor also play a role in renal disease models. Therefore, in the present study, we have examined the renal profile of Ang-(1-7), ACE2 and the Mas receptor in renal I/R and compared them with that of AngII, ACE and the AT(1) receptor. Male Wistar rats were submitted to left nephrectomy and ischaemia (45 min) followed by reperfusion (2 or 4 h) in the right kidney. At 4 h of reperfusion, renal AngII was increased (P < 0.01) and renal Ang-(1-7) was decreased substantially (P < 0.05), although plasma levels of both angiotensins were unchanged. in addition, renal I/R decreased the renal mRNA expression of renin (P < 0.05), AT(1) receptors (P < 0.001) and ACE2 (P < 0.05). At 2 and 4 h of reperfusion, renal ACE activity was reduced (P < 0.05). On the other hand, renal expression of the Mas receptor was greatly increased at 4 h of reperfusion (P < 0.01), which was confirmed by immunohistochemical and Western blot analysis. in conclusion, increased renal expression of the Mas receptor associated with changes in the RAS (renin-angiotensin-system)-related peptidases support an important role for the ACE2 Ang-(1-7) Mas axis in AKI.
Keywords acute kidney injury
angiotensin I-converting enzyme (ACE)
angiotensin I-converting enzyme 2 (ACE2)
angiotensin-(1-7) [Ang-(1-7)]
angiotensin II (AngII)
Mas receptor
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Grant number CAPES: PRDEX2009
CNPq: 8701480/1997-4
FAPEMIG: CBS 2044/96
Date 2010-11-01
Published in Clinical Science. London: Portland Press Ltd, v. 119, n. 9-10, p. 385-394, 2010.
ISSN 0143-5221 (Sherpa/Romeo, impact factor)
Publisher Portland Press Ltd
Extent 385-394
Origin http://dx.doi.org/10.1042/CS20090554
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000284130000003
URI http://repositorio.unifesp.br/handle/11600/33007

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