Inflammation and adipose tissue: effects of progressive load training in rats

Inflammation and adipose tissue: effects of progressive load training in rats

Author Lira, Fabio Santos de Autor UNIFESP Google Scholar
Rosa Neto, José Cesar Autor UNIFESP Google Scholar
Pimentel, Gustavo Duarte Autor UNIFESP Google Scholar
Tarini, Victor Alexandre Ferreira Autor UNIFESP Google Scholar
Arida, Ricardo Mario Autor UNIFESP Google Scholar
Faloppa, Flavio Autor UNIFESP Google Scholar
Alves, Eduardo da Silva Autor UNIFESP Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Seelaender, Marilia Google Scholar
Mello, Marco Tulio de Autor UNIFESP Google Scholar
Santos, Ronaldo Vagner Thomatieli dos Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Introduction: Cytokines (IL-6, IL-10 and TNF-alpha) are increased after exhaustive exercise in the rat retroperitoneal (RPAT) and mesenteric adipose tissue (MEAT) pads. On the other hand, these cytokines show decreased expression in these depots in response to a chronic exercise protocol. However, the effect of exercise with overload combined with a short recovery period on pro-and anti-inflammatory cytokine expression is unknown. in the present study, we investigated the regulation of cytokine production in the adipose tissue of rats after an overtraining-inducing exercise protocol.Methods: Male Wistar rats were divided into four groups: Control (C), Trained (Tr), Overtrained (OT) and recovered overtrained (R). Cytokines (IL-6, TNF-alpha and IL-10) levels and Toll Like Receptor 4 (TLR4), Nuclear Factor kBBp65 (NF-kBp65), Hormone Sensitive Lipase (HSL) and, Perilipin protein expression were assessed in the adipose tissue. Furthermore, we analysed plasma lipid profile, insulin, testosterone, corticosterone and endotoxin levels, and liver triacylglycerol, cytokine content, as well as apolipoprotein B (apoB) and TLR4 expression in the liver.Results: OT and R groups exhibited reduced performance accompanied by lower testosterone and increased corticosterone and endotoxin levels when compared with the control and trained groups. IL-6 and IL-10 protein levels were increased in the adipose tissue of the group allowed to recover, in comparison with all the other studied groups. TLR-4 and NF-kBp65 were increased in this same group when compared with both control and trained groups. the protein expression of HSL was increased and that of Perilipin, decreased in the adipose in R in relation to the control. in addition, we found increased liver and serum TAG, along with reduced apoB protein expression and IL-6 and IL-10 levels in the of R in relation to the control and trained groups.Conclusion: in conclusion, we have shown that increases in pro-inflammatory cytokines in the adipose tissue after an overtraining protocol may be mediated via TLR-4 and NF-kBp65 signalling, leading to an inflammatory state in this tissue.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2008/03533-1
Date 2010-10-04
Published in Lipids in Health and Disease. London: Biomed Central Ltd, v. 9, 10 p., 2010.
ISSN 1476-511X (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 10
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000283174700001

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