Riboflavin and photoproducts in MC3T3-E1 differentiation

Riboflavin and photoproducts in MC3T3-E1 differentiation

Author Chaves Neto, Antonio Hernandes Google Scholar
Yano, Claudia Lumy Google Scholar
Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Machado, Daisy Google Scholar
Justo, Giselle Zenker Autor UNIFESP Google Scholar
Peppelenbosch, Maikel P. Google Scholar
Ferreira, Carmen Verissima Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Univ Groningen
Abstract Photoderivatives of riboflavin can modulate the proliferation and survival of cancer cells. in this work, we examined the influence of riboflavin and photoderivatives on osteoblast differentiation induced by ascorbic acid and beta-glycerophosphate. These compounds decreased the osteoblast proliferation, increased the alkaline phosphatase activity, promoted a reduction in matrix metalloproteinase-2 activity and the decreased in the OPG/RANKL ratio. the effects of flavins on osteoblasts were unrelated to the antioxidant activity of these compounds. the biological activity of osteogenic medium containing riboflavin and its photoderivatives involved the activation of different signaling pathways (AKT, FAK, CaMKII), caspases-3, -8 and -9, and up-regulation of the expression and/or stabilization of osteoblastic transcription factors (Runx2 and beta-catenin). These findings suggest a potential use of flavins as adjuvants to improve bone metabolism. (C) 2010 Elsevier B.V. All rights reserved.
Keywords Ascorbic acid
Differentiation
beta-Glycerophosphate
Osteoblast
Riboflavin
Signaling
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 06/00430-1
Date 2010-10-01
Published in Toxicology in Vitro. Oxford: Pergamon-Elsevier B.V., v. 24, n. 7, p. 1911-1919, 2010.
ISSN 0887-2333 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1911-1919
Origin http://dx.doi.org/10.1016/j.tiv.2010.07.026
Access rights Closed access
Type Article
Web of Science ID WOS:000283567500005
URI http://repositorio.unifesp.br/handle/11600/32961

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