Fructose alters adiponectin, haptoglobin and angiotensinogen gene expression in 3T3-L1 adipocytes

Fructose alters adiponectin, haptoglobin and angiotensinogen gene expression in 3T3-L1 adipocytes

Author Carvalho, Carla R. Autor UNIFESP Google Scholar
Bueno, Allain A. Autor UNIFESP Google Scholar
Mattos, Ana M. Autor UNIFESP Google Scholar
Biz, Carolina Autor UNIFESP Google Scholar
Oliveira, Cristiane de Autor UNIFESP Google Scholar
Pisani, Luciana P. Autor UNIFESP Google Scholar
Ribeiro, Eliane B. Autor UNIFESP Google Scholar
Oller do Nascimento, Claudia M. Autor UNIFESP Google Scholar
Oyama, Lila M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Fructose- or sucrose-rich diets can cause insulin resistance and increase the risk of cardiovascular disease. Adipokines are correlated with the development of these diseases in obesity. We hypothesize that fructose and sucrose induce insulin resistance via effects on adipokine gene expression in adipocytes. This study analyzed the effect of fructose or glucose on adiponectin, haptoglobin, and angiotensinogen gene expression in 3T3-L1 adipocytes. Ten days after differentiation, the cells were pretreated with serum- and glucose-free medium. Twenty-four hours later, fructose or glucose (0, 5, 10, or 20 mmol) was added into the medium, and the cells were collected after a further 24 hours. Adiponectin, haptoglobin, and angiotensinogen gene expression were determined. Adiponectin gene expression increased when 10 or 20 mmol glucose was added compared with that observed for the non hexose-treated cells. A similar effect occurred when 5 mmol fructose was added. Glucose (10 mmol) and fructose (20 mmol) stimulated haptoglobin gene expression in 3T3-L1 adipocytes compared with 0 mmol, with glucose producing a more pronounced effect. Although 20 mmol fructose caused an increase in angiotensinogen gene expression, glucose did not. in conclusion, in this study of 2 hexoses revealed an increase in adiponectin gene expression, suggesting that the effect of a glucose-rich diet on the development of insulin resistance is not related to the effect of these hexoses on adipocyte adiponectin gene expression. However, insulin resistance and cardiovascular disease promoted by fructose-rich diets could be partially related to the effect of fructose on adiponectin and angiotensinogen gene expression. (C) 2010 Elsevier Inc. All rights reserved.
Keywords Glucose
Fructose
Adiponectin
Haptoglobin
Angiotensinogen
3T3-L1 adipocyte
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2010-09-01
Published in Nutrition Research. Oxford: Pergamon-Elsevier B.V., v. 30, n. 9, p. 644-649, 2010.
ISSN 0271-5317 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 644-649
Origin http://dx.doi.org/10.1016/j.nutres.2010.06.012
Access rights Closed access
Type Article
Web of Science ID WOS:000283334800008
URI http://repositorio.unifesp.br/handle/11600/32881

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