Impairment of the mitochondrial electron transport chain due to sleep deprivation in mice

Impairment of the mitochondrial electron transport chain due to sleep deprivation in mice

Author Andreazza, Ana C. Google Scholar
Andersen, Monica L. Autor UNIFESP Google Scholar
Alvarenga, Tathiana A. Autor UNIFESP Google Scholar
de-Oliveira, Marcos R. Google Scholar
Armani, Fernanda Autor UNIFESP Google Scholar
Ruiz, Francieli S. Autor UNIFESP Google Scholar
Giglio, Larriany Google Scholar
Moreira, Jose C. F. Google Scholar
Kapczinski, Flavio Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Institution Univ Fed Rio Grande do Sul
Univ British Columbia
Universidade Federal de São Paulo (UNIFESP)
Abstract It has been demonstrated that sleep deprivation is associated with altered expression of genes related to metabolic processes, response to stress and inflammation, circadian sleep/wake cycles, regulation of cell proliferation and various signaling pathways. However, the molecular mechanisms underlying these changes remain poorly understood. Thus, the present study aims to characterize the function of the mitochondrial electron transport chain in the brain using an animal model of paradoxical sleep deprivation (PSD). the question of whether sleep recovery (rebound) can reverse changes found after PSD is also addressed. Adult male inbred C57BL/6 J mice were randomly distributed into three groups: home-cage control, PSD and sleep rebound groups. the PSD and rebound groups were subjected to PSD for 72 h. After this sleep deprivation period, the rebound group was returned to its home cage and allowed to sleep in an undisturbed and spontaneous fashion for 24 h. the mitochondrial complex complex II, succinate dehydrogenase and complex II-III activities were then measured by spectrophotometric methods in sub-mitochondrial particles extracted from the prefrontal cortex, hippocampus, striatum and hypothalamus. Our results showed a significant decrease in the activity of complex in the PSD and rebound groups as compared to the control group. the complex II and II-III activity were particularly decreased in the hypothalamus of the sleep rebound group. These results are consistent with the involvement of sleep in energy metabolism and corroborate previous experiments demonstrating the importance of the hypothalamus in sleep regulation. (C) 2010 Elsevier B.V. All rights reserved.
Keywords Sleep deprivation
Rebound
Mitochondrial electron transport chain
Oxidative stress
Mice
Language English
Sponsor Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 98/14303-3
FAPESP: 09/01030-5
Date 2010-09-01
Published in Journal of Psychiatric Research. Oxford: Pergamon-Elsevier B.V., v. 44, n. 12, p. 775-780, 2010.
ISSN 0022-3956 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 775-780
Origin http://dx.doi.org/10.1016/j.jpsychires.2010.01.015
Access rights Closed access
Type Article
Web of Science ID WOS:000281330100006
URI http://repositorio.unifesp.br/handle/11600/32877

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