Raman spectroscopy for differential diagnosis of endophthalmitis and uveitis in rabbit iris in vitro

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dc.contributor.author Rossi, Eglas Emanuel
dc.contributor.author Silveira, Landulfo
dc.contributor.author Barbosa Pinheiro, Antonio Luiz
dc.contributor.author Zamuner, Stella Regina
dc.contributor.author Aimbire, Flavio
dc.contributor.author Maia, Mauricio [UNIFESP]
dc.contributor.author Tavares Pacheco, Marcos Tadeu
dc.date.accessioned 2016-01-24T14:05:25Z
dc.date.available 2016-01-24T14:05:25Z
dc.date.issued 2010-09-01
dc.identifier http://dx.doi.org/10.1016/j.exer.2010.06.005
dc.identifier.citation Experimental Eye Research. London: Academic Press Ltd- Elsevier B.V., v. 91, n. 3, p. 362-368, 2010.
dc.identifier.issn 0014-4835
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32873
dc.description.abstract We developed a diagnostic tool to differentiate between endophthalmitis and uveitis using Raman spectroscopy. Twenty-two New Zealand rabbits with endophthalmitis induced by Staphylococcus aureus (10 animals), noninfectious uveitis induced by lipopolysaccharide from Escherichia colt (10 animals) and controls (two animals) were analyzed. Twenty-four hours after the eyes were inoculated, iris tissue was dissected and subjected to dispersive Raman spectroscopy using an excitation source at 830 nm and a spectrograph/CCD camera to detect a Raman signal with an integration time of 50 s. With the collected spectra of endophthalmitis and uveitis, we developed a routine to classify spectra in each specimen using principal components analysis, using a leave-one-out cross-validation procedure. the mean Raman spectra of tissues with uveitis and endophthalmitis showed several bands in the region of 800-1800 cm(-1), which have been attributed to nucleic acids, amino acids, proteins, and lipids. the bands at 1004, 1339, and 1555 cm(-1) differed significantly (t-test. p < 0.05) between diseases. the principal components PC3 and PC4 differed significantly (ANOVA, p < 0.05) for the two tissue types, indicating that these PCs can be used to discriminate between the two diseases using Mahalanobis distance as a discriminator. This technique is useful for differentiating the spectral bands of uveitis and endophthalmitis, and the diagnostic model showed sensitivity of 89%, specificity of 100%, and accuracy of 92% using the leave-one-out cross-validation procedure. These results may be clinically relevant for differentiating endophthalmitis from uveitis, and this approach may become a noninvasive method to optimize the diagnosis of inflammatory and infectious vitreoretinal diseases. (C) 2010 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 362-368
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Experimental Eye Research
dc.rights Acesso restrito
dc.subject Raman spectroscopy en
dc.subject iris en
dc.subject endophthalmitis en
dc.subject uveitis en
dc.subject differential diagnosis en
dc.subject principal components analysis en
dc.subject Mahalanobis distance en
dc.subject discriminant analysis en
dc.title Raman spectroscopy for differential diagnosis of endophthalmitis and uveitis in rabbit iris in vitro en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Univ Comunitaria Reg Chapeco UNOCHAPECO
dc.contributor.institution Univ Camilo Castelo Branco UNICASTELO
dc.contributor.institution Universidade Federal da Bahia (UFBA)
dc.contributor.institution Nove Julho Univ UNINOVE
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Comunitaria Reg Chapeco UNOCHAPECO, Hlth Sci Ctr CCS, BR-89809000 Chapeco, SC, Brazil
dc.description.affiliation Univ Camilo Castelo Branco UNICASTELO, Nucleo Parque Tecnol Sao Jose dos Campos, Ctr Biomed Engn, BR-12247004 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliation Univ Fed Bahia, Sch Dent, BR-40110150 Salvador, BA, Brazil
dc.description.affiliation Nove Julho Univ UNINOVE, Rehabil Sci Masters Program, BR-05001100 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo UNIFESP, Vitreoretinal Surg Unit, Dept Ophthalmol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP, Vitreoretinal Surg Unit, Dept Ophthalmol, BR-04023062 São Paulo, Brazil
dc.description.sponsorshipID CNPq: 305610/2008-2
dc.description.sponsorshipID CNPq: 302444/2005-0
dc.identifier.doi 10.1016/j.exer.2010.06.005
dc.description.source Web of Science
dc.identifier.wos WOS:000281500700006


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