Collagen Changes and Realignment Induced by Low-Level Laser Therapy and Low-Intensity Ultrasound in the Calcaneal Tendon

Collagen Changes and Realignment Induced by Low-Level Laser Therapy and Low-Intensity Ultrasound in the Calcaneal Tendon

Author Wood, Viviane T. Autor UNIFESP Google Scholar
Pinfildi, Carlos E. Google Scholar
Neves, Marco A. I. Google Scholar
Parizoto, Nivaldo A. Google Scholar
Hochman, Bernardo Google Scholar
Ferreira, Lydia M. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de São Carlos (UFSCar)
Abstract Background and Objective: the treatment of calcaneal tendon injuries requires long-term rehabilitation Ultrasound (US) and low-level laser therapy (LLLT) are the most used and studied physical agents in the treatment of tendon injuries, however, only a few studies examined the effects of the combination of US and LLLT. Therefore, the purpose of this study was to investigate which treatment (the exclusive or combined use of US and LLLT) most effectively contribute to tendon healing.Study Design/Materials and Methods: This was a controlled laboratory study with 50 rats whose Achilles tendon was injured by direct trauma. the rats were randomly divided into five groups and treated for 5 consecutive days, as follows: group 1 (control) received no treatment; group 2 was treated with US alone, group 3 was treated with LLLT alone, group 4 was treated first with US followed by LLLT, and group 5 was treated first with LLLT followed by US On the sixth post-injury day, the tendons were removed and examined by polarized light microscopy. the organization of collagen fibers was assessed by birefringence measurements Picrosirius-stained sections were examined for the presence of types I and Ill collagen.Results: There was a significantly higher organization of collagen fibers in group 2 (US) than in the control group (P=0.03). the amount of type I collagen found in groups 2 (US), 3 (LLLT), and 5 (LLLT+US) was significantly higher than that in the control group (P <= 0.01), but no significant differences were found between treatment groups. There were no differences in the amount of type III collagen between groups.Conclusion: Ultrasound, LLLT, and the combined use of LLLT and US resulted in greater synthesis of type I collagen; US was also effective in increasing collagen organization in the early stages of the healing process Lasers Surg. Med. 42 559-565, 2010 (C) 2010 Wiley-Liss, Inc.
Keywords achilles tendon
birefringence
diode laser
physical therapy
picrosirius red
tendon injuries
ultrasonography
Language English
Sponsor Division of Plastic Surgery of the Federal University of São Paulo (UNIFESP)
Laboratory of Vitreous Materials (LaMaV)
Department of Materials of the Federal University of Sao Carlos (UFSCar)
Bioset Industria de Tecnologia Eletronica Ltda
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2010-08-01
Published in Lasers in Surgery and Medicine. Hoboken: Wiley-liss, v. 42, n. 6, p. 559-565, 2010.
ISSN 0196-8092 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 559-565
Origin http://dx.doi.org/10.1002/lsm.20932
Access rights Closed access
Type Article
Web of Science ID WOS:000281009400015
URI http://repositorio.unifesp.br/handle/11600/32762

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