Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains

Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains

Author Kahan, Vanessa Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Andersen, Monica Levy Autor UNIFESP Google Scholar
Alvarenga, Tathiana Aparecida Autor UNIFESP Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The purpose of the present study was to determine the genetic damage induced by paradoxical sleep deprivation (PSD) in three different male mice strains in peripheral blood, heart, kidney and liver tissues by the single cell gel (comet) assay. Swiss, C57BL/6j and hairless (HRS/j) mice were submitted to PSD by the multiple platform technique for 72 hr, and DNA damage was evaluated. Statistically significant differences in DNA damage were found in blood cells of the Swiss mice strain when compared to negative controls. By contrast, no statistically significant differences were found in the C57BL/6j or hairless mice strains. With regard to the liver, extensive genotoxic effects were found in the Swiss strain. the hairless and C57BL/6j mice strains did not show any signs of genotoxocity in this organ. the same lack of effect was noted in kidney and heart cells of all strains evaluated. in conclusion, our results reveal that sleep deprivation exerted genetic damage in the form of DNA breakage in blood and liver cells of the Swiss mice strain only. This type of approach should be considered when studying noxious activities on genetic apparatus induced by sleep deprivation in mice since the Swiss strain is more suitable for this purpose.
Language English
Sponsor Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 06/58274-5
FAPESP: 07/01228-4
FAPESP: CEPID 98/14303-3
Date 2010-07-01
Published in Basic & Clinical Pharmacology & Toxicology. Malden: Wiley-Blackwell, v. 107, n. 1, p. 598-602, 2010.
ISSN 1742-7835 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 598-602
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000278931000008

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