Effect of endometriosis on the protein expression pattern of follicular fluid from patients submitted to controlled ovarian hyperstimulation for in vitro fertilization

Effect of endometriosis on the protein expression pattern of follicular fluid from patients submitted to controlled ovarian hyperstimulation for in vitro fertilization

Author Lo Turco, Edson Guimaraes Autor UNIFESP Google Scholar
Martins Ferreira Souza, Gustavo Henrique Google Scholar
Garcia, Jerusa Simone Google Scholar
Ferreira, Christina Ramires Google Scholar
Eberlin, Marcos Nogueira Google Scholar
Bertolla, Ricardo Pimenta Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Waters Corp
Universidade Estadual de Campinas (UNICAMP)
Alfenas Fed Univ
Abstract BACKGROUND: the aim of this study was to evaluate protein expression profile and quantify the proteins present in follicular fluid (FF) samples from women with endometriosis and pregnant women without endometriosis.METHODS: A prospective case control study was carried out including women with Stage III or IV endometriosis (Group I) and pregnant women without endometriosis (Group II), both at the maximum age of 35 years. Women were submitted to controlled ovarian stimulation for in vitro fertilization, and FF was collected after ultrasound-guided ovarian aspiration. FF from both ovaries was pooled, and patient samples were pooled according to Group I or II. Pooled protein samples were separated and analyzed by MudPIT (multidimensional protein identification technology followed by Expression(E) and label-free quantification with ProteinLynxGlobalServer 2.4v, Identity(E) and Expression(E) software).RESULTS: A total of 416 proteins or randomic sequence were identified, 62 proteins differentially expressed between Groups I and II. One ( 1.6%) was expressed at a higher level and 36 (58.1%) were uniquely expressed in Group 1, whereas 8 (12.9%) were expressed at a higher level and 17 (27.4%) were uniquely expressed in Group II. of all these, 15 (24.2%) are related to binding, I (1.6%) to immune response, 8 (12.9%) to cell division, 3 (4.8%) to cellular metabolism, 16 (25.8%) to general function and 19 (30.6%) do not yet present an identified function.CONCLUSIONS: Protein expression profiles of patients with and without endometriosis identified at least 64 proteins differentially expressed, which may be related to the physiopathology of endometriosis. These proteins may additionally be useful in determining potential biomarkers for diagnostics, as well as for therapeutic intervention in women with infertility due to endometriosis.
Keywords biomarkers
endometriosis
IVF
follicular fluid
proteomics
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Division of Urology, Human Reproduction Section at the São Paulo Federal University
ThoMSon Mass Spectrometry Laboratory of the Institute of Chemistry at the University of Campinas
Grant number CNPq: 475 108/2009-4
FAPESP: 2008/10756-7
Date 2010-07-01
Published in Human Reproduction. Oxford: Oxford Univ Press, v. 25, n. 7, p. 1755-1766, 2010.
ISSN 0268-1161 (Sherpa/Romeo, impact factor)
Publisher Oxford Univ Press
Extent 1755-1766
Origin http://dx.doi.org/10.1093/humrep/deq102
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000280142800019
URI http://repositorio.unifesp.br/handle/11600/32641

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