Acute exercise reverses aged-induced impairments in insulin signaling in rodent skeletal muscle

Acute exercise reverses aged-induced impairments in insulin signaling in rodent skeletal muscle

Author Pauli, Jose R. Autor UNIFESP Google Scholar
Ropelle, Eduardo R. Google Scholar
Cintra, Dennys Esper Google Scholar
De Souza, Claudio Teodoro Google Scholar
Silva, Adelino S. R. da Google Scholar
Moraes, Juliana C. Google Scholar
Prada, Patricia O. Google Scholar
Almeida Leme, Jose A. C. de Google Scholar
Luciano, Eliete Google Scholar
Velloso, Licio A. Google Scholar
Carvalheira, Jose B. C. Google Scholar
Saad, Mario J. A. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Univ Extremo Sul Catarinense
Abstract The insulin resistance associated with aging is improved by exercise, but the molecular mechanisms of this improvement are not fully understood. We investigated whether the improvement in insulin action, associated with acute exercise in old rats is dependent on the modulation of pIRS-1Ser307, JNK, IkB alpha and PIP-1B. Aging rats were subjected to swimming for two 1.5-h long bouts, separated by a 45 min rest period. Sixteen hours after the exercise, the rats were killed and proteins from the insulin signaling pathway were analyzed by immunoblotting. Our results show that the reduction in glucose disappearance rate (Kitt), observed in aged rats, was restored at 16 h after exercise. Aging led to an increase in Ser307 phosphorylation of IRS-1, and this was reversed by exercise in the skeletal muscle, in parallel with a reduction in pJNK and IkB alpha degradation. Moreover, aging induced an increase in the expression of PTP-1B and attenuated insulin signaling in the muscle of rats, a phenomenon that was reversed by exercise. Interestingly, the decrease in PTP-1B expression in the muscle of exercised old rats was accompanied by an increase in SIRT1 expression. These results provide new insights into the mechanisms by which exercise restores insulin sensitivity during aging. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
Keywords Aging
Acute exercise
Insulin signaling and inflammation
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2006/06961-9
FAPESP: 2006/06960-2
Date 2010-05-01
Published in Mechanisms of Ageing and Development. Clare: Elsevier B.V., v. 131, n. 5, p. 323-329, 2010.
ISSN 0047-6374 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 323-329
Access rights Closed access
Type Article
Web of Science ID WOS:000279163800004

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