Single embryo and oocyte lipid fingerprinting by mass spectrometry

Single embryo and oocyte lipid fingerprinting by mass spectrometry

Author Ferreira, Christina R. Google Scholar
Saraiva, Sergio A. Google Scholar
Catharino, Rodrigo R. Google Scholar
Garcia, Jerusa S. Google Scholar
Gozzo, Fabio C. Google Scholar
Sanvido, Gustavo B. Google Scholar
Santos, Luiz Fernando A. Google Scholar
Lo Turco, Edson G. Autor UNIFESP Google Scholar
Pontes, Jose Henrique F. Google Scholar
Basso, Andrea C. Google Scholar
Bertolla, Ricardo P. Autor UNIFESP Google Scholar
Sartori, Roberto Google Scholar
Guardieiro, Monique M. Google Scholar
Perecin, Felipe Google Scholar
Meirelles, Flvio V. Google Scholar
Sangalli, Juliano R. Google Scholar
Eberlin, Marcos N. Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Vitro Brasil Ltda
Universidade de São Paulo (USP)
Abstract Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid ( represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species.-Ferreira, C. R., S. A. Saraiva, R. R. Catharino, J. S. Garcia, F. C. Gozzo, G. B. Sanvido, L. F. A. Santos, E. G. Lo Turco, J. H. F. Pontes, A. C. Basso, R. P. Bertolla, R. Sartori, M. M. Guardieiro, F. Perecin, F. V. Meirelles, J. R. Sangalli, and M. N. Eberlin. Single embryo and oocyte lipid fingerprinting by mass spectrometry. J. Lipid Res. 2010. 51: 1218-1227.
Keywords oocyte
mass spectrometry
lipid fingerprinting
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2008/10756-7
Date 2010-05-01
Published in Journal of Lipid Research. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 51, n. 5, p. 1218-1227, 2010.
ISSN 0022-2275 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Biochemistry Molecular Biology Inc
Extent 1218-1227
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000276633100038

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