Phosphoproteome Reveals an Atlas of Protein Signaling Networks During Osteoblast Adhesion

Phosphoproteome Reveals an Atlas of Protein Signaling Networks During Osteoblast Adhesion

Author Milani, Renato Google Scholar
Ferreira, Carmen V. Google Scholar
Granjeiro, Jose M. Google Scholar
Paredes-Gamero, Edgar J. Autor UNIFESP Google Scholar
Silva, Rodrigo A. Google Scholar
Justo, Giselle Z. Autor UNIFESP Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Galembeck, Eduardo Google Scholar
Peppelenbosch, Maikel P. Google Scholar
Aoyama, Hiroshi Google Scholar
Zambuzzi, Willian F. Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal Fluminense (UFF)
Universidade Federal de São Paulo (UNIFESP)
Univ Groningen
Abstract Cell adhesion on surfaces is a fundamental process in the emerging biomaterials field and developmental events as well. However, the mechanisms regulating this biological process in osteoblasts are not fully understood. Reversible phosphorylation catalyzed by kinases is probably the most important regulatory mechanism in eukaryotes. Therefore, the goal of this study is to assess osteoblast adhesion through a molecular prism under a peptide array technology, revealing essential signaling proteins governing adhesion-related events. First, we showed that there are main morphological changes on osteoblast shape during adhesion up to 3 h. Second, besides classical proteins activated upon integrin activation, our results showed a novel network involving signaling proteins such as Rap1A, PKA, PKC, and GSK3 beta during osteoblast adhesion on polystyrene. Third, these proteins were grouped in different signaling cascades including focal adhesion establishment, cytoskeleton rearrangement, and cell-cycle arrest. We have thus provided evidence that a global phosphorylation screening is able to yield a systems-oriented look at osteoblast adhesion, providing new insights for understanding of bone formation and improvement of cell-substratum interactions. Altogether, these statements are necessary means for further intervention and development of new approaches for the progress of tissue engineering. Cell. Biochem. 109: 957-966, 2010. (C) 2010 Wiley-Liss, Inc.
Keywords OSTEOBLAST
CELL ADHESION
SIGNAL TRANSDUCTION
SURFACES
KINOME
PKC
RAP1
BIOENGINEERING
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 08/53003-9
Date 2010-04-01
Published in Journal of Cellular Biochemistry. Hoboken: Wiley-liss, v. 109, n. 5, p. 957-966, 2010.
ISSN 0730-2312 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 957-966
Origin http://dx.doi.org/10.1002/jcb.22479
Access rights Closed access
Type Article
Web of Science ID WOS:000276418900015
URI http://repositorio.unifesp.br/handle/11600/32399

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