Presence of mRNA of muscle nicotinic acetylcholine receptor subunits and an epsilon-subunit splice variant in the mouse brain

Presence of mRNA of muscle nicotinic acetylcholine receptor subunits and an epsilon-subunit splice variant in the mouse brain

Author Ghedini, P. C. Autor UNIFESP Google Scholar
Honda, L. Autor UNIFESP Google Scholar
Avellar, M. C. W. Autor UNIFESP Google Scholar
Souccar, C. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Transcripts encoding for alpha 1, beta 1, delta, gamma and epsilon (and its splice variant epsilon(S)) subunits of the muscle-type nicotinic acetylcholine receptor (nAChR) were assessed using reverse transcription followed by polymerase chain reaction (RT-PCR) assays, with RNA extracted from the mouse skeletal muscle (diaphragm) and brain regions (cortex, hippocampus and cerebellum). the presence of alpha 1, beta 1, delta, gamma, epsilon and epsilon(S) transcripts was confirmed in the diaphragm muscle, used as positive control. mRNAs coding for muscle alpha 1, beta 1, delta, epsilon, epsilon(S), but not gamma subunits, were detected in adult mouse brain regions. An epsilon-subunit sequence variant, named epsilon(t), was also detected in all brain regions examined, but not in skeletal muscle. This new epsilon-subunit splice variant lacks a 115 bp cassette corresponding to exon 8 in the first intracellular transmembrane domain of the subunit, leading to a truncated protein. the data provide evidence for the presence of muscle-type nAChR subunits in the mouse central nervous system. (C) 2009 Elsevier Inc. All Fights reserved.
Keywords Nicotinic acetylcholine receptor
RT-PCR
Splice variant
Skeletal muscle
Central nervous system
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2010-03-16
Published in Brain Research Bulletin. Oxford: Pergamon-Elsevier B.V., v. 81, n. 4-5, p. 453-457, 2010.
ISSN 0361-9230 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 453-457
Origin http://dx.doi.org/10.1016/j.brainresbull.2009.12.006
Access rights Closed access
Type Article
Web of Science ID WOS:000274972800015
URI http://repositorio.unifesp.br/handle/11600/32381

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