STORAGE RESULTS in LOSS of the ANTIGENOTOXIC PROPERTIES of LENTINULA EDODES (SHIITAKE MUSHROOM) and DEVELOPMENT of in VIVO GENOTOXICITY

STORAGE RESULTS in LOSS of the ANTIGENOTOXIC PROPERTIES of LENTINULA EDODES (SHIITAKE MUSHROOM) and DEVELOPMENT of in VIVO GENOTOXICITY

Author Alves de Lima, Patricia L. Google Scholar
Ribeiro, Daniel A. Autor UNIFESP Google Scholar
Sugui, Marina M. Google Scholar
Terezan, Ana Paula Google Scholar
Domingues, Maria Aparecida C. Google Scholar
Filho, E. R. Google Scholar
Azevedo, Luciana Google Scholar
Salvadori, Daisy M. F. Google Scholar
Ribeiro, Lucia R. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de São Carlos (UFSCar)
Univ Fed Alfenas
Abstract In this study, we have investigated the stability of the antigenotoxic properties of dried of Lentinula edodes (Berk.) Singer mushroom powder stored at room and at -20C. Mice were pretreated by gavage for 15 consecutive days with 0.6 mL/day of an aqueous extract of dried L. edodes powder prepared at 60C. On day 15, the mice were intraperitoneally injected with 50 mg/kg N-ethyl-N-nitrosourea (ENU); 3 h later, peripheral blood was withdrawn from the animals and used to evaluate DNA damage using the comet assay. the mice were sacrificed 24 h after the ENU treatment and the bone marrow used to measure the frequency of micronucleated polychromatic erythrocytes. the results indicated that storage at both room temperature and at -20C significantly reduced the antigenotoxic effects of the mushroom powder. in addition, whereas freshly dried mushroom powder was nongenotoxic, storage of the powder at -20C resulted in genotoxic activity (P < 0.05). Taken together, our data indicate that constituents of L. edodes powder are altered during storage, resulting in both genotoxicity and a reduction in antigenotoxicity.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2010-02-01
Published in Journal of Food Biochemistry. Malden: Wiley-Blackwell Publishing, Inc, v. 34, n. 1, p. 206-228, 2010.
ISSN 0145-8884 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 206-228
Origin http://dx.doi.org/10.1111/j.1745-4514.2009.00275.x
Access rights Closed access
Type Article
Web of Science ID WOS:000274200900016
URI http://repositorio.unifesp.br/handle/11600/32254

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