Are endogenous sex hormones related to DNA damage in paradoxically sleep-deprived female rats?

Are endogenous sex hormones related to DNA damage in paradoxically sleep-deprived female rats?

Author Andersen, Monica L. Autor UNIFESP Google Scholar
Ribeiro, Daniel A. Autor UNIFESP Google Scholar
Alvarenga, Tathiana A. Autor UNIFESP Google Scholar
Silva, Andressa Autor UNIFESP Google Scholar
Araujo, Paula Autor UNIFESP Google Scholar
Zager, Adriano Autor UNIFESP Google Scholar
Tenorio, Neuli M. Autor UNIFESP Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of this investigation was to evaluate overall DNA damage induced by experimental paradoxical sleep deprivation (PSD) in estrous-cycling and ovariectomized female rats to examine possible hormonal involvement during DNA damage. Intact rats in different phases of the estrous cycle (proestrus, estrus, and diestrus) or ovariectomized female Wistar rats were subjected to PSD by the single platform technique for 96 h or were maintained for the equivalent period as controls in home-cages. After this period, peripheral blood and tissues (brain, liver, and heart) were collected to evaluate genetic damage using the single cell gel (comet) assay. the results showed that PSD caused extensive genotoxic effects in brain cells, as evident by increased DNA migration rates in rats exposed to PSD for 96 h when compared to negative control. This was observed for all phases of the estrous cycle indistinctly. in ovariectomized rats, PSD also led to DNA damage in brain cells. No significant statistically differences were detected in peripheral blood, the liver or heart for all groups analyzed. in conclusion, our data are consistent with the notion that genetic damage in the form of DNA breakage in brain cells induced by sleep deprivation overrides the effects related to endogenous female sex hormones. (c) 2009 Elsevier Inc. All rights reserved.
Keywords Sleep deprivation
DNA damage
Progesterone
Testosterone
Corticosterone
Hormones
Ovariectomy
Estrous cycle
Single cell gel (comet) assay
Female rats
Language English
Sponsor Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 98/14303-3
FAPESP: 07/01228-4
FAPESP: 09/01030-5
FAPESP: 07/566206
FAPESP: 06/58275-1
Date 2010-02-01
Published in Hormones and Behavior. San Diego: Academic Press Inc Elsevier Science, v. 57, n. 2, p. 216-221, 2010.
ISSN 0018-506X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 216-221
Origin http://dx.doi.org/10.1016/j.yhbeh.2009.11.004
Access rights Closed access
Type Article
Web of Science ID WOS:000274434100017
URI http://repositorio.unifesp.br/handle/11600/32237

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