Alterations in Calcium Signaling and a Decrease in Bcl-2 Expression: Possible Correlation With Apoptosis in Aged Striatum

Alterations in Calcium Signaling and a Decrease in Bcl-2 Expression: Possible Correlation With Apoptosis in Aged Striatum

Author Ureshino, R. P. Autor UNIFESP Google Scholar
Bertoncini, C. R. Autor UNIFESP Google Scholar
Fernandes, M. J. S. Google Scholar
Abdalla, F. M. F. Google Scholar
Porto, C. S. Autor UNIFESP Google Scholar
Hsu, Y-T. Google Scholar
Lopes, G. S. Autor UNIFESP Google Scholar
Smaili, Soraya Soubhi Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Butantan Inst
Med Univ S Carolina
Abstract Aging is a multifaceted process associated with various functional and structural deficits that might be evolved in degenerative diseases. It has been shown that neurodegenerative disorders are associated with alterations in Ca(2+) homeostasis. Thus, in the present work, we have investigated Ca(2+) signaling and apoptosis in aged striatum. Our results show that glutamate and NMDA evoke a greater Ca(2+) rise in striatum slices from aged animals. However, this difference is not present when glutamate is tested in the absence of external Ca(2+). Immunostaining of glutamate receptors shows that only NMDA receptors (NRI) are increased in the striatum of aged rats. Increases in mitochondrial Ca(2+) content and in the reactive oxygen species levels were also observed in aged animals, which could be associated with tissue vulnerability. in addition, a decrease in the Bcl-2 protein expression and an enhancement in apoptosis were also present in aged striatum. Together the results indicate that, in aged animals, alterations in Ca(2+) handling coupled to an increase in ROS accumulation and a decrease in the prosurvival protein Bcl-2 may contribute to apoptosis induction and cell death in rat striatum. (C) 2009 Wiley-Liss, Inc.
Keywords aging
striatum
glutamate
calcium
apoptosis
Bcl-2
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
NIH
Grant number NIH: NS 40932
Date 2010-02-01
Published in Journal of Neuroscience Research. Hoboken: Wiley-liss, v. 88, n. 2, p. 438-447, 2010.
ISSN 0360-4012 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 438-447
Origin http://dx.doi.org/10.1002/jnr.22214
Access rights Closed access
Type Article
Web of Science ID WOS:000273361600021
URI http://repositorio.unifesp.br/handle/11600/32204

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