Transcervical hysteroscopic sterilization using cyanoacrylate: A long-term experimental study on sheep

Transcervical hysteroscopic sterilization using cyanoacrylate: A long-term experimental study on sheep

Author Bigolin, Sergio Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Rivoire, Henri Chaplin Google Scholar
Negrini Fagundes, Anna Tereza Autor UNIFESP Google Scholar
Negrini Fagundes, Anna Luiza Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Santo Amaro
Reg Integrated Univ High Uruguay & Missions
Rio Grande Fed Univ
Abstract Aim:We investigated the transcervical hysteroscopy application of n-butyl-2-cyanoacrylate in the uterine tube lumen of a non-rodent animals (sheep) with fallopian tube dimensions similar those in humans.Methods:Standard hysteroscopic procedures were performed on female Texel sheep (n = 26). the right and left ostia were identified. for each ewe, a urethral catheter (5Fr) was used for the delivery of 0.5 mL of saline or an equal volume of n-butyl-2-cyanoacrylate into the uterine tube. Following the procedure, ewes were housed with males of proven fertility for 90 days (equivalent to 5.5 estrous cycles). Postmortem (dye and burst pressure) and in vivo (hysterosalpingogram) testing for tube patency were both performed 90 days and 180 days following the procedure.Results:All animals receiving the saline treatment became pregnant. Gross inspection of uterine tubes following n-butyl-2-cyanoacrylate treatment revealed no visceral adhesions or fibrosis. However, postmortem testing revealed total obstruction within the fallopian tubes. This was confirmed by hysterosalpingogram, in that iodine contrast did not escape into the abdominal cavity.Conclusion:The cyanoacrylate promoted a reliable fallopian tube obstruction without fibrosis in an animal model exhibiting a similar tube diameter to that found in women. the technique can be evaluated for efficacy in vivo using hysterosalpingography.
Keywords experimental surgery
reproductive sterilization
surgical adhesive
uterine tube
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2009-12-01
Published in Journal of Obstetrics and Gynaecology Research. Malden: Wiley-Blackwell Publishing, Inc, v. 35, n. 6, p. 1012-1018, 2009.
ISSN 1341-8076 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1012-1018
Access rights Closed access
Type Article
Web of Science ID WOS:000272344200002

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