Influence of colloid particle profile on sentinel lymph node uptake

Influence of colloid particle profile on sentinel lymph node uptake

Author Fernandez Nunez, Eutimio Gustavo Google Scholar
Faintuch, Bluma Linkowski Google Scholar
Teodoro, Rodrigo Google Scholar
Wiecek, Danielle Pereira Google Scholar
Martinelli, Jose Roberto Google Scholar
Silva, Natanael Gomes da Google Scholar
Castanheira, Claudia E. Google Scholar
Oliveira Filho, Renato Santos de Autor UNIFESP Google Scholar
Pasqualini, Roberto Google Scholar
Institution Inst Energet & Nucl Res
Universidade Federal de São Paulo (UNIFESP)
CIS Bio Int
Abstract Introduction: Particle size of colloids employed for sentinel lymph node (LN) detection is not well studied. This investigation aimed to correlate particle size and distribution of different products with LN uptake.Methods: All agents (colloidal tin, dextran, phytate and colloidal rhenium sulfide) were labeled with (99m)Tc according to manufacturer's instructions. Sizing of particles was carried out oil electron micrographs using Image Tool for Windows (Version 2.0). Biodistribution studies in main excretion organs as well as in popliteal LN were Performed in male Wistar rats [30 and 90 min post injection (p.i.)]. the injected dose was 0.1 ml (37 MBq) in the footpad of the left posterior limb. Dynamic images (0-15 min p.i.) as well as static ones (30 and 90 min) were acquired in gamma camera.Results: Popliteal LN was clearly reached by all products. Nevertheless, particle size remarkably influenced node uptake. Colloidal rhenium sulfide, with the smallest diameter (5.1 x 10(-3)+/- 3.9x10(-3) mu m), permitted the best result [2.72 +/- 0.64 percent injected dose (%ID) at 90 min]. Phytate displayed small particles (<15 mu m) with favorable uptake (1.02 +/- 0.14%ID). Dextran (21.4 +/- 12.8 mu m) and colloidal tin (39.0 +/- 8.3 mu m) were less effective (0.55 +/- 0.14 and 0.06 +/- 0.03%ID respectively). Particle distribution also tended to influence results. When asymmetric, it was associated with biphasic uptake which increased over time; conversely, symmetric distribution (colloidal tin) was consistent with a constant pattern.Conclusion: the results are suggesting that particle size and symmetry may interfere with LN radiopharmaceutical uptake. (C) 2009 Elsevier Inc. All rights reserved.
Keywords Technetium colloids
sentinel lymph node
(99m)Tc-dextran
(99m)Tc-phytate
(99m)Tc-colloidal tin
(99m)Tc-colloidal rhenium sulfide
Language English
Date 2009-10-01
Published in Nuclear Medicine and Biology. New York: Elsevier B.V., v. 36, n. 7, p. 741-747, 2009.
ISSN 0969-8051 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 741-747
Origin http://dx.doi.org/10.1016/j.nucmedbio.2009.04.009
Access rights Closed access
Type Article
Web of Science ID WOS:000269852700005
URI http://repositorio.unifesp.br/handle/11600/31863

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