A study of human furin specificity using synthetic peptides derived from natural substrates, and effects of potassium ions

A study of human furin specificity using synthetic peptides derived from natural substrates, and effects of potassium ions

Author Izidoro, Mario A. Autor UNIFESP Google Scholar
Gouvea, Iuri E. Autor UNIFESP Google Scholar
Santos, Jorge A. N. Autor UNIFESP Google Scholar
Assis, Diego M. Autor UNIFESP Google Scholar
Oliveira, Vitor Autor UNIFESP Google Scholar
Judice, Wagner A. S. Autor UNIFESP Google Scholar
Juliano, Maria A. Autor UNIFESP Google Scholar
Lindberg, Iris Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Maryland
Abstract We explored furin Substrate requirements in addition to the motif R-X-K/R-R using synthetic fluorescent resonance energy transfer (FRET) decapeptides. These decapeptides were derived from furin cleavage sites in viral coat glycoproteins and human and bacterial protein precursors. the hydrolysis by furin of most substrate was activated by K(+) ion, whereas kosmotropic anions of the Hofmeister series were inhibitors. the analysis of furin hydrolytic activity showed that its efficiency is highly dependent on the particular combinations of amino acids at different Substrate positions. There is a clear interdependence of furin subsites that must be taken in account in determining its specificity and also for the design of inhibitors. However, clear preferences were detected for Substrates with S at P'(1), and V at P'(2), at P'(3) the amino acids D, S, 1, and A are almost equally frequent. in the non-prime subsites the best Substrates presented S and H at P(6); basic amino acids at P(5); and no clear tendency at P(3). Interestingly, two amino acid substitutions on the prime side of the peptide derived from H5N1 influenza hemagglutinin furin processing site highly improved its hydrolysis. These modifications are possible by single point Mutations, Suggesting a potential yield of a more infectious virus. (C) 2009 Elsevier Inc. All rights reserved.
Keywords Protease
Peptidase
Proprotein convertase
Language English
Date 2009-07-15
Published in Archives of Biochemistry and Biophysics. New York: Elsevier B.V., v. 487, n. 2, p. 105-114, 2009.
ISSN 0003-9861 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 105-114
Origin http://dx.doi.org/10.1016/j.abb.2009.05.013
Access rights Closed access
Type Article
Web of Science ID WOS:000268072500005
URI http://repositorio.unifesp.br/handle/11600/31675

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