Disrupted Cell Cycle Control in Cultured Endometrial Cells from Patients with Endometriosis Harboring the Progesterone Receptor Polymorphism PROGINS

Disrupted Cell Cycle Control in Cultured Endometrial Cells from Patients with Endometriosis Harboring the Progesterone Receptor Polymorphism PROGINS

Author D'Amora, Paulo Autor UNIFESP Google Scholar
Maciel, Thiago Trovati Autor UNIFESP Google Scholar
Tambellini, Rodrigo Autor UNIFESP Google Scholar
Mori, Marcelo A. Autor UNIFESP Google Scholar
Pesquero, João Bosco Autor UNIFESP Google Scholar
Sato, Helio Autor UNIFESP Google Scholar
Girão, Manoel João Batista Castello Autor UNIFESP Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Schor, Eduardo Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Presently, little is understood about how endometriosis is established or maintained, or how genetic factors can predispose women to the disease. Because of the crucial role that the progesterone receptor polymorphism PROGINS plays in predisposing women to the development of endometriosis, we hypothesized that this variant may influence critical steps during endometrial cell metabolism that are involved in the pathogenesis of endometriosis. Eutopic endometria were collected from three sources: women with endometriosis who had a single PROGINS allele (from the progesterone receptor gene); women with endometriosis who had the wild-type progesterone receptor allele; and women without endometriosis who had the wild-type allele. Cells prepared from the eutopic endometria of these women were stimulated with both estradiol and progesterone, and then examined for cell proliferation, viability, and apoptosis. the cells from women with endometriosis that carried the PROGINS allele demonstrated increased proliferation, greater viability, and decreased apoptosis following progesterone treatment. in general, these parameters were very different as compared with those of women with endometriosis but without the PROGINS allele and women in the control group. This result indicates there is a reduced level of progesterone responsiveness in women who carry the PROGINS polymorphism. Because progesterone responsiveness is known to be an important characteristic of women with endometriosis, these data support the contention that the PROGINS polymorphism enhances the endometriosis phenotype. (Am J Pathol 2009, 175:215-224; DOI: 10.2353/ajpath.2009.080966)
Language English
Date 2009-07-01
Published in American Journal of Pathology. Bethesda: Amer Soc Investigative Pathology, Inc, v. 175, n. 1, p. 215-224, 2009.
ISSN 0002-9440 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Investigative Pathology, Inc
Extent 215-224
Origin http://dx.doi.org/10.2353/ajpath.2009.080966
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000267508600020
URI http://repositorio.unifesp.br/handle/11600/31626

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