Effect of Low-Level Laser Therapy on Mast Cells in Viability of the Transverse Rectus Abdominis Musculocutaneous Flap

Effect of Low-Level Laser Therapy on Mast Cells in Viability of the Transverse Rectus Abdominis Musculocutaneous Flap

Author Pinfildi, Carlos Eduardo Autor UNIFESP Google Scholar
Liebano, Richard Eloin Autor UNIFESP Google Scholar
Hochman, Bernardo Autor UNIFESP Google Scholar
Enokihara, Milvia M. M. S. S. Autor UNIFESP Google Scholar
Lippert, Rodrigo Google Scholar
Gobbato, Rafael Correa Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Objective: To assess the effect of low-level laser therapy (LLLT) on viability of mast cells of the transverse rectus abdominis musculocutaneous (TRAM) flap. Background Data: LLLT has been recently used on the TRAM flap to stimulate mast cells. Materials and Methods: Eighty-four Wistar rats were randomly divided into seven groups of 12 rats in each: group 1 (sham laser therapy); group 2 received 3 J/cm(2) at one point; group 3 received 3 J/cm(2) at 24 points; group 4 received 72 J/cm(2) at 1 point; group 5 received 6 J/cm2 at 1 point; group 6 received 6 J/cm(2) at 24 points; and group 7 received 144 J/cm(2) at 1 point. All experimental groups underwent LLLT immediately after TRAM surgery and on the next two following days, for three sessions in total. the percentage of the area of skin flap necrosis was calculated on the fourth postoperative day and two samples of skin were collected from each rat with a 1-cm(2) punch to perform mast cell evaluations with toluidine blue dye. Results: Statistically significant differences were found in the percentage of necrosis, and higher values were seen in group 1 than in all other groups. Among groups 3-7 no statistically significant differences were found (p < 0.292). for mast cells, when group 1 was compared to groups 5 (6 J/cm(2) at 1 point) and 7 (144 J/cm(2) at 1 point), it had fewer mast cells. Conclusion: LLLT at a wavelength of 670 nm was effective at reducing the necrotic area, and we found that it can stimulate mast cells growth to increase vascular perfusion.
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2009-04-01
Published in Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc, v. 27, n. 2, p. 337-343, 2009.
ISSN 1549-5418 (Sherpa/Romeo, impact factor)
Publisher Mary Ann Liebert Inc
Extent 337-343
Origin http://dx.doi.org/10.1089/pho.2008.2295
Access rights Closed access
Type Article
Web of Science ID WOS:000265363800017
URI http://repositorio.unifesp.br/handle/11600/31437

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