Different Intervals of Ischemic Preconditioning on Small Bowel Ischemia-Reperfusion Injury in Rats

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dc.contributor.author Jacome, D. T.
dc.contributor.author Abrahao, M. S.
dc.contributor.author Morello, Ricardo José [UNIFESP]
dc.contributor.author Martins, Jose Luiz [UNIFESP]
dc.contributor.author Medeiros, A. C.
dc.contributor.author Montero, E. F. S. [UNIFESP]
dc.date.accessioned 2016-01-24T13:52:25Z
dc.date.available 2016-01-24T13:52:25Z
dc.date.issued 2009-04-01
dc.identifier http://dx.doi.org/10.1016/j.transproceed.2009.01.071
dc.identifier.citation Transplantation Proceedings. New York: Elsevier B.V., v. 41, n. 3, p. 827-829, 2009.
dc.identifier.issn 0041-1345
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31427
dc.description.abstract Purpose. the purpose of this study was to establish morphologically the best time of vascular occlusion to induce ischemic preconditioning (IPC) for rat small bowel undergoing ischemia and reperfusion injury.Methods. After approval by the Ethics Committee, 36 EPM-1 young adult Wistar rats from 300-350 g were distributed into 6 groups: sham (S); ischemia and reperfusion (IR), with 50 minutes of cranial mesenteric artery occlusion and 30 minutes of reperfusion; IPC with 1 cycle of 2 minutes (IPC-2), 5 minutes (IPC-5), 10 minutes (IPC-10), or 15 minutes (IPC-15), followed by sustained IR. the animals anesthetized with ketamine (60 mg/kg) and xylazine (10 mg/kg) intramuscular (IM), were maintained on mattress heat, hydrated with saline (80 mL/kg), and injected with 100 IU heparin. Samples of jejunum were stained with hematoxylin and eosin (HE) and classified according to Park et al. Statistical analysis of results was performed using Kruskal-Wallis tests (P <.05).Results. the histological evaluation showed no difference between IR and IPC15 rats (5.2 and 5, respectively; P =.84). Greater jejunal injury was observed with IPC15 (5) compared with other groups (IPC2 = 3, P =.03; IPC5 = 3.2, P =.05; IPC10 = 2.8, P = .02, respectively). There was no difference between groups IPC2 X IPC5 X IPCIO.Conclusion. Morphologically, IPC with short times promoted greater intestinal protection against the IR lesion in rats. en
dc.format.extent 827-829
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Transplantation Proceedings
dc.rights Acesso restrito
dc.title Different Intervals of Ischemic Preconditioning on Small Bowel Ischemia-Reperfusion Injury in Rats en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Univ Fed Rio Grande do Norte
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Rio Grande do Norte, Dept Surg, BR-59072970 Natal, RN, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Surg & Res Postgraduating Program, Div Pediat Surg, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Surg, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Surg & Res Postgraduating Program, Div Pediat Surg, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Surg, São Paulo, Brazil
dc.identifier.doi 10.1016/j.transproceed.2009.01.071
dc.description.source Web of Science
dc.identifier.wos WOS:000265504300012



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