Blockade of Bradykinin Receptor B1 but Not Bradykinin Receptor B2 Provides Protection From Cerebral Infarction and Brain Edema

Blockade of Bradykinin Receptor B1 but Not Bradykinin Receptor B2 Provides Protection From Cerebral Infarction and Brain Edema

Author Austinat, Madeleine Google Scholar
Braeuninger, Stefan Google Scholar
Pesquero, João Bosco Autor UNIFESP Google Scholar
Brede, Marc Google Scholar
Bader, Michael Autor UNIFESP Google Scholar
Stoll, Guido Google Scholar
Renne, Thomas Google Scholar
Kleinschnitz, Christoph Google Scholar
Institution Univ Wurzburg
Universidade Federal de São Paulo (UNIFESP)
Max Delbruck Ctr Mol Med
Abstract Background and Purpose-Brain edema is detrimental in ischemic stroke and its treatment options are limited. Kinins are proinflammatory peptides that are released during tissue injury. the effects of kinins are mediated by 2 different receptors (B1 and B2 receptor [B1R and B2R]) and comprise induction of edema formation and release of proinflammatory mediators.Methods-Focal cerebral ischemia was induced in B1R knockout, B2R knockout, and wild-type mice by transient middle cerebral artery occlusion. Infarct volumes were measured by planimetry. Evan's blue tracer was applied to determine the extent of brain edema. Postischemic inflammation was assessed by real-time reverse-transcriptase polymerase chain reaction and immunohistochemistry. To analyze the effect of a pharmacological kinin receptor blockade, B1R and B2R inhibitors were injected.Results-B1R knockout mice developed significantly smaller brain infarctions and less neurological deficits compared to wild-type controls (16.8 +/- 4.7 mm(3) vs 50.1 +/- 9.1 mm(3), respectively; P < 0.0001). This was accompanied by a dramatic reduction of brain edema and endothelin-1 expression, as well as less postischemic inflammation. Pharmacological blockade of B1R likewise salvaged ischemic tissue (15.0 +/- 9.5 mm(3) vs 50.1 +/- 9.1 mm(3), respectively; P < 0.01) in a dose-dependent manner, even when B1R inhibitor was applied 1 hour after transient middle cerebral artery occlusion. in contrast, B2R deficiency did not confer neuroprotection and had no effect on the development of tissue edema.Conclusions-These data demonstrate that blocking of B1R can diminish brain infarction and edema formation in mice and may open new avenues for acute stroke treatment in humans. (Stroke. 2009; 40: 285-293.)
Keywords bradykinin
edema
endothelin-1
inflammation
stroke
Language English
Sponsor Interdisciplinary Clinical Research Center (IZKF) Wurzburg
Date 2009-01-01
Published in Stroke. Philadelphia: Lippincott Williams & Wilkins, v. 40, n. 1, p. 285-293, 2009.
ISSN 0039-2499 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 285-293
Origin http://dx.doi.org/10.1161/STROKEAHA.108.526673
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000262059400047
URI http://repositorio.unifesp.br/handle/11600/31118

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