Administration of Neural Precursor Cells Ameliorates Renal Ischemia-Reperfusion Injury

Show simple item record

dc.contributor.author Wang, Pamella Huey Mei [UNIFESP]
dc.contributor.author Schwindt, Telma Tiemi [UNIFESP]
dc.contributor.author Barnabe, Gabriela Filoso [UNIFESP]
dc.contributor.author Motta, Fabiana Louise Teixeira [UNIFESP]
dc.contributor.author Semedo, Patricia [UNIFESP]
dc.contributor.author Beraldo, Felipe Caetano
dc.contributor.author Mazzali, Marilda
dc.contributor.author Reis, Marlene Antonia dos
dc.contributor.author Teixeira, Vicente de Paula Antunes
dc.contributor.author Pacheco-Silva, Alvaro [UNIFESP]
dc.contributor.author Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]
dc.contributor.author Camara, Niels Olsen Saraiva [UNIFESP]
dc.date.accessioned 2016-01-24T13:52:00Z
dc.date.available 2016-01-24T13:52:00Z
dc.date.issued 2009-01-01
dc.identifier http://dx.doi.org/10.1159/000210575
dc.identifier.citation Nephron Experimental Nephrology. Basel: Karger, v. 112, n. 1, p. E20-E28, 2009.
dc.identifier.issn 1660-2129
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31117
dc.description.abstract In this study we evaluated whether administration of stem cells of neural origin (neural precursor cells, NPCs) could be protective against renal ischemia-reperfusion injury (IRI). We hypothesized that stem cell outcomes are not tissue-specific and that NPCs can improve tissue damage through paracrine mechanisms, especially due to immunomodulation. To this end, Wistar rats (200-250 g) were submitted to 1-hour ischemia and treated with NPCs (4 x 10(6) cells/animal) at 4 h of reperfusion. To serve as controls, ischemic animals were treated with cerebellum homogenate harvested from adult rat brain. All groups were sacrificed at 24 h of reperfusion. NPCs were isolated from rat fetus telencephalon and cultured until neurosphere formation (7 days). Before administration, NPCs were labeled with carboxyfluorescein diacetate succinimydylester (CFSE). Kidneys were harvested for analysis of cytokine profile and macrophage infiltration. At 24 h, NPC treatment resulted in a significant reduction in serum creatinine (IRI + NPC 1.21 + 0.18 vs. IRI 3.33 + 0.14 and IRI + cerebellum 2.95 + 0.78mg/dl, p < 0.05) and acute tubular necrosis (IRI + NPC 46.0 + 2.4% vs. IRI 79.7 + 14.2%, p < 0.05). NPC-CFSE and glial fibrillary acidic protein (GFAP)-positive cells (astrocyte marker) were found exclusively in renal parenchyma, which also presented GFAP and SOX-2 (an embryonic neural stem cell marker) mRNA expression. NPC treatment resulted in lower renal proinflammatory IL1-beta and TNF-alpha expression and higher anti-inflammatory IL-4 and IL-10 transcription. NPC-treated animals also had less macrophage infiltration and decreased serum proinflammatory cytokines (IL-1 beta, TNF-alpha and INF-gamma). Our data suggested that NPC therapy improved renal function by influencing immunological responses. Copyright (C) 2009 S. Karger AG, Basel en
dc.description.sponsorship MCT/CT-Saude/Decit/SCTIE/MS
dc.description.sponsorship Fundacao de Apoio a Pesquisa do Estado de São Paulo
dc.format.extent E20-E28
dc.language.iso eng
dc.publisher Karger
dc.relation.ispartof Nephron Experimental Nephrology
dc.rights Acesso aberto
dc.subject Neurospheres en
dc.subject Neural precursor cells en
dc.subject Ischemia and reperfusion injury, renal en
dc.subject Immunomodulation en
dc.title Administration of Neural Precursor Cells Ameliorates Renal Ischemia-Reperfusion Injury en
dc.type Artigo
dc.rights.license http://www.karger.com/Services/RightsPermissions
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Estadual de Campinas (UNICAMP)
dc.contributor.institution Univ Fed Triangulo Mineiro
dc.contributor.institution Inst Israelita Ensino & Pesquisa Albert Einstein
dc.description.affiliation Univ São Paulo, Dept Immunol, Inst Biomed Sci, Lab Transplantat Immunobiol, BR-05508900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Lab Imunol Clin & Expt, Div Nephrol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Neurophysiol Lab, Div Physiol, São Paulo, Brazil
dc.description.affiliation Univ Estadual Campinas, Div Nephrol, Campinas, SP, Brazil
dc.description.affiliation Univ Fed Triangulo Mineiro, Div Pathol, Belo Horizonte, MG, Brazil
dc.description.affiliation Inst Israelita Ensino & Pesquisa Albert Einstein, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Lab Imunol Clin & Expt, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Neurophysiol Lab, Div Physiol, São Paulo, Brazil
dc.description.sponsorshipID MCT/CT-Saude/Decit/SCTIE/MS: 552307/2005-0
dc.description.sponsorshipID Fundacao de Apoio a Pesquisa do Estado de São Paulo: 04/08311-6
dc.description.sponsorshipID Fundacao de Apoio a Pesquisa do Estado de São Paulo: 04/13826-5
dc.description.sponsorshipID Fundacao de Apoio a Pesquisa do Estado de São Paulo: 05/50085-6
dc.description.sponsorshipID Fundacao de Apoio a Pesquisa do Estado de São Paulo: 07/07139-3
dc.identifier.doi 10.1159/000210575
dc.description.source Web of Science
dc.identifier.wos WOS:000265628600003



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account