Expression of functional recombinant human factor IX in milk of mice

Expression of functional recombinant human factor IX in milk of mice

Author Lisauskas, Sharon F. C. Google Scholar
Cunha, Nicolau B. Google Scholar
Vianna, Giovanni R. Google Scholar
Mendes, Erica A. Google Scholar
Ramos, Gustavo L. Google Scholar
Maranhao, Andreia Q. Google Scholar
Brigido, Marcelo M. Google Scholar
Almeida, Jussara O. S. C. Google Scholar
Baptista, Heloisa A. Autor UNIFESP Google Scholar
Motta, Fabiana L. T. Autor UNIFESP Google Scholar
Pesquero, Joao B. Autor UNIFESP Google Scholar
Aragao, Francisco J. L. Google Scholar
Rech, Elibio L. Google Scholar
Institution Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade de Brasília (UnB)
SGAIN
Universidade Federal de São Paulo (UNIFESP)
Abstract Human factor IX is synthesized in the liver and secreted in the blood, where it participates in a group of reactions involving coagulation factors and proteins that permit sanguinary coagulation. in this work two lines of transgenic mice were developed to express the FIX gene in the mammalian glands under control of milk beta-casein promoter. the founding females secreted the FIX in their milk (3% total soluble protein). the stable integration of transgene was confirmed by southern blot analysis. the presence of the FIX recombinant protein in the milk of transgenic females was confirmed by western blot and the clotting activity was revealed in blood-clotting assays. the coagulation activity in human blood treated with recombinant FIX increased while the time of coagulation decreased. Our results confirm the production of a large amount of recombinant biologically active FIX in the mammary gland of transgenic mice.
Keywords Expression
Recombinant human factor IX
Transgenic mice
Language English
Sponsor Empresa Brasileira de Pesquisa Agropecuaria (Embrapa)
Hospital de Apoio de Brasilia
Universidade Federal de São Paulo
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
International Hemophilia Training Center-World Federation of Hemophilia
Date 2008-12-01
Published in Biotechnology Letters. Dordrecht: Springer, v. 30, n. 12, p. 2063-2069, 2008.
ISSN 0141-5492 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 2063-2069
Origin http://dx.doi.org/10.1007/s10529-008-9818-y
Access rights Closed access
Type Article
Web of Science ID WOS:000260306800004
URI http://repositorio.unifesp.br/handle/11600/31064

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