Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells

Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells

Author Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Leon, Carlos M. M. P. Autor UNIFESP Google Scholar
Borojevic, Radovan Google Scholar
Oshiro, Maria Etsuko Miyamoto Autor UNIFESP Google Scholar
Ferreira, Alice Teixeira Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Abstract The role of intracellular Ca2+ (Ca-i(2+)) on hematopoiesis was investigated in long term bone marrow cultures using cytokines and agonists of P2 receptors. Cytokines interleukin 3 and granulocyte/macrophage colony stimulator factor promoted a modest increase in Ca-i(2+) concentration ([Ca2+](i)) with activation of phospholipase C gamma, MEK1/2, and Ca2+/calmodulin kinase II. Involvement of protein kinase C was restricted to stimulation with interleukin 3. in addition, these cytokines promoted proliferation (20 times) and an increase in the Gr-1(-)Mac-1(+) population with participation of gap junctions (GJ). Nevertheless ATP, ADP, and UTP promoted a large increase in [Ca2+](i), moderate proliferation (6 times), a reduction in the primitive Gr-1(-)Mac-1(-)c-Kit(+) population, and differentiation into macrophages without participation of GJ. It is likely that Ca-i(2+) participates as a regulator of hematopoietic signaling: moderate increases in [Ca2+](i) would be related to cytokine-dependent proliferation with participation of GJ, whereas high increases in [Ca2+](i) would be related to macrophage differentiation without maintenance of the primitive population.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2008-11-14
Published in Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 283, n. 46, p. 31909-31919, 2008.
ISSN 0021-9258 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Biochemistry Molecular Biology Inc
Extent 31909-31919
Origin http://dx.doi.org/10.1074/jbc.M801990200
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000260760800073
URI http://repositorio.unifesp.br/handle/11600/31036

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account