Dietary fish oil did not prevent sleep deprived rats from a reduction in adipose tissue adiponectin gene expression

Dietary fish oil did not prevent sleep deprived rats from a reduction in adipose tissue adiponectin gene expression

Author Mattos, Ana Barbosa Marcondes de Autor UNIFESP Google Scholar
Pinto, Monica Jordao S. Autor UNIFESP Google Scholar
Oliveira, Cristiane Autor UNIFESP Google Scholar
Biz, Carolina Autor UNIFESP Google Scholar
Ribeiro, Eliane Beraldi Autor UNIFESP Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Andersen, Monica Levy Autor UNIFESP Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Sleep deprivation in humans has been related to weight gain and consequently, increased risk for insulin resistance. in contrast, there is a significant loss of weight in sleep deprived rats suggesting a state of insulin resistance without obesity interference. Thus, we aimed to assess the effects of a rich fish oil dietetic intervention on glucose tolerance, serum insulin and adiponectin, and adipose tissue gene expression of adiponectin and TNF-alpha of paradoxically sleep deprived (PSD) rats. the study was performed in thirty day-old male Wistar randomly assigned into two groups: rats fed with control diet ( soybean oil as source of fat) and rats fed with a fish oil rich diet. After 45 days of treatment, the animals were submitted to PSD or maintained as home cage control group for 96 h. Body weight and food intake were carefully monitored in all groups. At the end of PSD period, a glucose tolerance test was performed and the total blood and adipose tissues were collected. Serum insulin and adiponectin were analyzed. Adipose tissues were used for RT-PCR to estimate the gene expression of adiponectin and TNF-alpha. Results showed that although fish oil diet did not exert any effect upon these measurements, PSD induced a reduction in adiponectin gene expression of retroperitoneal adipose tissues, with no change in serum adiponectin concentration or in adiponectin and TNF-alpha gene expression of epididymal adipose tissue. Thus, the stress induced by sleep deprivation lead to a desbalance of adiponectin gene expression.
Language English
Date 2008-11-05
Published in Lipids in Health and Disease. London: Biomed Central Ltd, v. 7, 11 p., 2008.
ISSN 1476-511X (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 11
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000262253800001

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