IMPACT of CYTOMEGALOVIRUS and GRAFTS VERSUS HOST DISEASE ON the DYNAMICS of CD57+CD28-CD8+T CELLS AFTER BONE MARROW TRANSPLANT

IMPACT of CYTOMEGALOVIRUS and GRAFTS VERSUS HOST DISEASE ON the DYNAMICS of CD57+CD28-CD8+T CELLS AFTER BONE MARROW TRANSPLANT

Author Mendes, Ana Verena Almeida Google Scholar
Kallas, Esper Georges Autor UNIFESP Google Scholar
Benard, Gil Google Scholar
Pannuti, Claudio Sergio Google Scholar
Menezes, Renee Google Scholar
Dulley, Frederico Luiz Google Scholar
Evans, Thomas George Google Scholar
Salomao, Reinaldo Autor UNIFESP Google Scholar
Machado, Clarisse Martins Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVES: the present study aimed to evaluate the dynamics of CD28 and CD57 expression in CD8+ T lymphocytes during cytomegalovirus viremia in bone marrow transplant recipients.METHODS: in a prospective study, blood samples were obtained once weekly once from 33 healthy volunteers and weekly from 33 patients. To evaluate the expression of CD57 and CD28 on CD8+ T lymphocytes, flow cytometry analysis was performed on blood samples for four months after bone marrow transplant, together with cytomegalovirus antigenemia assays.RESULTS: Compared to cytomegalovirus-seronegative healthy subjects, seropositive healthy subjects demonstrated a higher percentage of CD57+ and a lower percentage of CD28+ cells (p < 0.05). A linear regression model demonstrated a continuous decrease in CD28+ expression and a continuous increase in CD57+ expression after bone marrow transplant. the occurrence of cytomegalovirus antigenemia was associated with a steep drop in the percentage of CD28+ cells (5.94%, p < 0.01) and an increase in CD57+ lymphocytes (5.60%, p < 0.01). This cytomegalovirus-dependent effect was for the most part concentrated in the allogeneic bone marrow transplant patients. the development of acute graft versus host disease, which occurred at an earlier time than antigenemia (day 26 vs. day 56 post-bone marrow transplant), also had an impact on the CD57+ subset, triggering an increase of 4.9% in CD57+ lymphocytes (p < 0.05).CONCLUSION: We found continuous relative changes in the CD28+ and CD57+ subsets during the first 120 days post-bone marrow transplant, as part of immune system reconstitution and maturation. A clear correlation was observed between the expansion of the CD57+CD28-CD8+ T lymphocyte subpopulation and the occurrence of graft versus host disease and cytomegalovirus viremia.
Keywords Cytomegalovirus
Graft versus host disease
Bone marrow transplant
CD57+lymphocytes
immuno-constitution
Language English
Sponsor University of São Paulo
Date 2008-10-01
Published in Clinics. São Paulo: Hospital Clinicas, Univ São Paulo, v. 63, n. 5, p. 667-676, 2008.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Hospital Clinicas, Univ São Paulo
Extent 667-676
Origin http://dx.doi.org/10.1590/S1807-59322008000500016
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000260168400015
SciELO ID S1807-59322008000500016 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/30926

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