Assessment of factors that confound MRI and neuropathological correlation of human postmortem brain tissue

Assessment of factors that confound MRI and neuropathological correlation of human postmortem brain tissue

Author Grinberg, Lea T. Google Scholar
Amaro, Edson Google Scholar
Teipel, Stefan Google Scholar
Santos, Denis Dionizio dos Google Scholar
Pasqualucci, Carlos Augusto Google Scholar
Leite, Renata E. P. Google Scholar
Camargo, Celia Regina Google Scholar
Goncalves, Jaqueline Alba Google Scholar
Sanches, Ariadne Goncalves Autor UNIFESP Google Scholar
Santana, Miriam Google Scholar
Ferretti, Renata E. L. Google Scholar
Jacob-Filho, Wilson Google Scholar
Nitrini, Ricardo Google Scholar
Heinsen, Helmut Google Scholar
Brazilian Aging Brain Study Grp Google Scholar
Institution Univ Wurzburg
Universidade de São Paulo (USP)
Inst Israelita Ensino & Pesquisa Albert Einstein
Univ Rostock
Universidade Federal de São Paulo (UNIFESP)
Abstract In spite of considerable technical advance in MRI techniques, the optical resolution of these methods are still limited. Consequently, the delineation of cytoarchitectonic fields based on probabilistic maps and brain volume changes, as well as small-scale changes seen in MRI scans need to be verified by neuronanatomical/neuropathological diagnostic tools. To attend the current interdisciplinary needs of the scientific community, brain banks have to broaden their scope in order to provide high quality tissue suitable for neuroimaging- neuropathology/anatomy correlation studies. the Brain Bank of the Brazilian Aging Brain Research Group (BBBABSG) of the University of São Paulo Medical School (USPMS) collaborates with researchers interested in neuroimaging-neuropathological correlation studies providing brains submitted to postmortem MRI in-situ. in this paper we describe and discuss the parameters established by the BBBABSG to select and to handle brains for fine-scale neuroimaging-neuropathological correlation studies, and to exclude inappropriate/unsuitable autopsy brains. We tried to assess the impact of the postmortem time and storage of the corpse on the quality of the MRI scans and to establish fixation protocols that are the most appropriate to these correlation studies. After investigation of a total of 36 brains, postmortem interval and low body temperature proved to be the main factors determining the quality of routine MRI protocols. Perfusion fixation of the brains after autopsy by mannitol 20% followed by formalin 20% was the best method for preserving the original brain shape and volume, and for allowing further routine and immunohistochemical staining. Taken to together, these parameters offer a methodological progress in screening and processing of human postmortem tissue in order to guarantee high quality material for unbiased correlation studies and to avoid expenditures by post-imaging analyses and histological processing of brain tissue.
Keywords brain
banking
postmortem
MRI
neuropathology
confounder
Language English
Date 2008-09-01
Published in Cell and Tissue Banking. Dordrecht: Springer, v. 9, n. 3, p. 195-203, 2008.
ISSN 1389-9333 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 195-203
Origin http://dx.doi.org/10.1007/s10561-008-9080-5
Access rights Closed access
Type Article
Web of Science ID WOS:000257917900005
URI http://repositorio.unifesp.br/handle/11600/30877

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