The monoclonal antibody against the major diagnostic antigen of Paracoccidioides brasiliensis mediates immune protection in infected BALB/c mice challenged intratracheally with the fungus

The monoclonal antibody against the major diagnostic antigen of Paracoccidioides brasiliensis mediates immune protection in infected BALB/c mice challenged intratracheally with the fungus

Author Buissa-Filho, R. Google Scholar
Puccia, Rosana Autor UNIFESP Google Scholar
Marques, A. F. Google Scholar
Pinto, F. A. Google Scholar
Munoz, J. E. Google Scholar
Nosanchuk, J. D. Google Scholar
Travassos, L. R. Autor UNIFESP Google Scholar
Taborda, Carlos Pelleschi Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Albert Einstein Coll Med
Abstract The protective role of specific antibodies against Paracoccidioides brasiliensis is controversial. in the present study, we analyzed the effects of monoclonal antibodies on the major diagnostic antigen (gp43) using in vitro and in vivo P. brasiliensis infection models. the passive administration of some monoclonal antibodies (MAbs) before and after intratracheal or intravenous infections led to a reduced fungal burden and decreased pulmonary inflammation. the protection mediated by MAb 3E, the most efficient MAb in the reduction of fungal burden, was associated with the enhanced phagocytosis of P. brasiliensis yeast cells by J774.16, MH-S, or primary macrophages. the ingestion of opsonized yeast cells led to an increase in NO production by macrophages. Passive immunization with MAb 3E induced enhanced levels of gamma interferon in the lungs of infected mice. the reactivity of MAb 3E against a panel of gp43-derived peptides suggested that the sequence NHVRIPIGWAV contains the binding epitope. the present work shows that some but not all MAbs against gp43 can reduce the fungal burden and identifies a new peptide candidate for vaccine development.
Language English
Date 2008-07-01
Published in Infection and Immunity. Washington: Amer Soc Microbiology, v. 76, n. 7, p. 3321-3328, 2008.
ISSN 0019-9567 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 3321-3328
Origin http://dx.doi.org/10.1128/IAI.00349-08
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000257172300055
URI http://repositorio.unifesp.br/handle/11600/30747

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