Chronic stress may modulate periodontal disease: A study in rats

Chronic stress may modulate periodontal disease: A study in rats

Author Peruzzo, Daiane C. Google Scholar
Benatti, Bruno B. Google Scholar
Antunes, Isabela B. Autor UNIFESP Google Scholar
Andersen, Monica L. Autor UNIFESP Google Scholar
Sallum, Enilson A. Google Scholar
Casati, Marcio Z. Google Scholar
Nociti, Francisco H. Google Scholar
Nogueira-Filho, Getulio R. Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Bahia Sci Fdn
Abstract Background: the present study aimed to evaluate whether chronic stress (CS) affects ligature-induced periodontal disease and to investigate the impact of CS on the mRNA levels of interleukin (IL)-1 beta, -1 receptor antagonist, -6, and -10, interferon-gamma (IFN-gamma), receptor activator of nuclear factor-kappa B ligand (RANKL), and osteoprotegerin in the gingival tissues of rats.Methods: Sixty male Wistar rats were assigned randomly to three groups: G1 (control; non-ligated sites), G2 (periodontal disease), and G3 (periodontal disease associated with restraint stress for 12 hours/day for the entire study). After 30 days, all animals were sacrificed by decapitation. Blood samples were taken, and the concentrations of corticosterone and catecholamines were measured as biomarkers of CS. Marginal tissues around ligated and non-ligated teeth were harvested, and gene expression was assessed by quantitative polymerase chain reaction. Moreover, the area of bone loss (ABL) was determined histometrically.Results: Data analysis showed that CS increased serum levels of stress biomarkers (P <0.05), ligature placement resulted in a significant ABL compared to non-ligated sites, CS significantly increased the amount of ABL in inflamed sites (P <0.001), and CS significantly increased mRNA levels of proinflammatory (ILAP and -6 and IFN-gamma) and anti-inflammatory (IL-10) cytokines and proresorptive factor (RANKL) in ligated sites (P<0.05).Conclusion: CS significantly increased bone loss resulting from ligature-induced periodontitis by a local increase in proinflammatory and proresorptive factors.
Keywords animal studies
chronic stress
inflammation
periodontal disease
Language English
Date 2008-04-01
Published in Journal of Periodontology. Chicago: Amer Acad Periodontology, v. 79, n. 4, p. 697-704, 2008.
ISSN 0022-3492 (Sherpa/Romeo, impact factor)
Publisher Amer Acad Periodontology
Extent 697-704
Origin http://dx.doi.org/10.1902/jop.2008.070369
Access rights Closed access
Type Article
Web of Science ID WOS:000255423000018
URI http://repositorio.unifesp.br/handle/11600/30589

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