Trypanosoma cruzi heparin-binding proteins and the nature of the host cell heparan sulfate-binding domain

Trypanosoma cruzi heparin-binding proteins and the nature of the host cell heparan sulfate-binding domain

Author Rodrigues de Oliveira, Francisco Odenclo Google Scholar
Alves, Carlos Roberto Google Scholar
Calvet, Claudia Magalhaes Google Scholar
Toma, Leny Autor UNIFESP Google Scholar
Boucas, Rodrigo Ippolito Autor UNIFESP Google Scholar
Nader, Helena Bociani Autor UNIFESP Google Scholar
Castro Cortes, Luzia Monteiro de Google Scholar
Krieger, Marco Aurelio Google Scholar
Meirelles, Maria de Nazareth S. L. Google Scholar
Souza Pereira, Mirian Claudia de Google Scholar
Institution Fiocruz MS
Universidade Federal de São Paulo (UNIFESP)
Abstract Trypanosoma cruzi invasion is mediated by receptor-ligand recognition between the surfaces of both parasite and target cell. We have previously demonstrated the role of heparan sulfate proteoglycan in the attachment and invasion of T cruzi in cardiomyocytes. Herein, we have isolated the T cruzi heparin-binding proteins (HBP-Tc) and investigated the nature of cardiomyocyte heparan Sulfate (HS)-binding site to the parasite surface ligand. Two major heparin-binding proteins with molecular masses of 65.8 and 59 kDa were observed in total extract of amastigote and trypomastigote forms of T cruzi. Hydrophobic [S(35)]methionine labeled proteins eluted from heparin-sepharose rose affinity chromatography also revealed both proteins in trypomastigotes but only the 59 kDa is strongly recognized by biotin-conjugated glycosaminoglycans. Competition assays were performed to analyze the role of sulfated proteoglycans, including heparin, keratan Sulfate and both acetylated and highly sulfated domains of heparan sulfate, in the recognition and invasion process of T cruzi. Significant inhibitions of 84% and 35% in the percentage of infection were revealed after treatment of the parasites with heparin and the N-acetylated/ N-sulfated heparan sulfate domain, respectively, Suggesting the important role of the glycuronic acid and NS glucosamine domain of the HS chain in the recognition of the HBP-Tc during the T cruzi-cardiomyocyte interaction. (C) 2007 Elsevier B.V. All rights reserved.
Keywords Trypanosoma cruzi
Cardiomyocytes
Glycosaminoglycans
Recognition process
Chromatography
Language English
Sponsor Fundac do Oswaldo Cruz (FIOCRUZ)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Programa Estrategico de Apoio a Pesquisa ern Saude (PAPES IV)
Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2008-04-01
Published in Microbial Pathogenesis. London: Academic Press Ltd Elsevier B.V., v. 44, n. 4, p. 329-338, 2008.
ISSN 0882-4010 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 329-338
Origin http://dx.doi.org/10.1016/j.micpath.2007.10.003
Access rights Closed access
Type Article
Web of Science ID WOS:000262591000008
URI http://repositorio.unifesp.br/handle/11600/30569

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