Kinin-B2 receptor expression and activity during differentiation of embryonic rat neurospheres

Kinin-B2 receptor expression and activity during differentiation of embryonic rat neurospheres

Author Martins, Antonio Henrique Baccin Autor UNIFESP Google Scholar
Alves, Janaina Maria Autor UNIFESP Google Scholar
Trujillo, Cleber A. Google Scholar
Schwindt, Telma Tiemi Autor UNIFESP Google Scholar
Barnabe, Gabriela Filooso Autor UNIFESP Google Scholar
Motta, Fabiana Louise Teixeira Autor UNIFESP Google Scholar
Guimarães, Alessander de Oliveira Autor UNIFESP Google Scholar
Casarini, Dulce Elena Autor UNIFESP Google Scholar
Mello, Luiz Eugenio Araujo de Moraes Autor UNIFESP Google Scholar
Pesquero, Joao Bosco Autor UNIFESP Google Scholar
Ulrich, Henning Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Neural progenitor cells were isolated from rat fetal telencephalon and proliferate as neurospheres in the presence of EGF, FGF-2, and heparin. in the absence of these growth factors, neurospheres differentiate into neurons, astrocytes, and oligodendrocytes. Using an embryonal carcinoma cell line as in vitro differentiation model, we have already demonstrated the presence of an autocrine loop system between kinin-B2 receptor activity and secretion of its ligand bradykinin (BK) as prerequisites for final neuronal differentiation (Martins et al., J Biol Chem 2005; 280: 19576-19586). the aim of this study was to verify the activity of the kallikrein-kinin system (KKS) during neural progenitor cell differentiation. Immunofluorescence studies and flow cytometry analysis revealed increases in glial fibrillary acidic protein and beta-3 tubulin expression and decrease in the number of nestin-positive cells along neurospheres differentiation, indicating the transition of neural progenitor cells to astrocytes and neurons. Kinin-B2 receptor expression and activity, secretion of BK into the medium, and presence of high-molecular weight kininogen suggest the participation of the KKS in neurosphere differentiation. Functional kinin-B2 receptors and BK secretion indicate an autocrine loop during neurosphere differentiation to neurons, astrocytes, and oligodendrocytes, reflecting events occurring during early brain development. (C) 2008 International Society for Analytical Cytology.
Keywords kinin-B2 receptor
neural differentiation
neurosphere
kallikrein-kinin system
Language English
Date 2008-04-01
Published in Cytometry Part A. Hoboken: Wiley-liss, v. 73A, n. 4, p. 361-368, 2008.
ISSN 1552-4922 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 361-368
Origin http://dx.doi.org/10.1002/cyto.a.20519
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000254699000012
URI http://repositorio.unifesp.br/handle/11600/30534

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