Mechanisms underlying the nociceptive and inflammatory responses induced by trypsin in the mouse paw

Mechanisms underlying the nociceptive and inflammatory responses induced by trypsin in the mouse paw

Author Paszcuk, Ana Flavia Google Scholar
Quintao, Nara L. M. Google Scholar
Fernandes, Elizabeth S. Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Chapman, Kevin Google Scholar
Andrade-Gordon, Patricia Google Scholar
Campos, Maria Martha Google Scholar
Vergnolle, Nathalie Google Scholar
Calixto, Joao B. Google Scholar
Institution Universidade Federal de Santa Catarina (UFSC)
Universidade Federal de São Paulo (UNIFESP)
Pontificia Univ Catolica Rio Grande do Sul
Univ Calgary
Abstract It has been demonstrated that trypsin is able to evoke the classical signals of inflammation, mainly via the activation of proteinase-activated receptor-2 (PAR-2). This study was designed to evaluate the inflammatory and nociceptive responses caused by trypsin injection in the mouse paw. Trypsin produced a dose- and time-related paw edema, a response that was markedly reduced in PAR-2-deficient mice compared to wild-type mice, particularly at the early time-points after trypsin injection. in addition, trypsin produced an increase in myeloperoxidase (MPO) activity, which was significantly reduced in PAR-2-deficient mice. the injection of trypsin into the mouse paw also elicited a dose- and time-dependent spontaneous nociception, as well as thermal and mechanical hypernociceptive responses, which were consistently decreased in mice with genetic deletion of PAR-2. Pharmacological evaluation revealed that edema formation and spontaneous nociception caused by trypsin injection in the mouse paw are mediated by a complex range of mediators. Both edema and nociception seem to rely on the production of neuropeptides, probably involving C-fibre activation and vanilloid receptor-1 (TRPV1), besides the stimulation of kinin B-2 receptors. Edematogenic response is also likely related to the production of cyclooxygenase (COX) metabolites, whereas the mast cell activation appears to be greatly associated to spontaneous nociception. Altogether, the present results indicate that trypsin-induced edema and nociception in the mouse paw represent multi-mediated responses that are largely, but not exclusively, related to the activation of PAR-2. These pieces of evidence provide new insights on the role of trypsin in pain and inflammation. (C) 2007 Elsevier B.V. All rights reserved.
Keywords trypsin
Language English
Date 2008-02-26
Published in European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 581, n. 1-2, p. 204-215, 2008.
ISSN 0014-2999 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 204-215
Access rights Closed access
Type Article
Web of Science ID WOS:000253575900025

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