Morphologic and clinical effects of subretinal injection of indocyanine green and infracyanine green in rabbits

Morphologic and clinical effects of subretinal injection of indocyanine green and infracyanine green in rabbits

Author Penha, Fernando M. Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Farah, Michel E. Autor UNIFESP Google Scholar
Dib, Eduardo Autor UNIFESP Google Scholar
Principe, Andre H. Autor UNIFESP Google Scholar
Devin, Fracois Google Scholar
Rodrigues, Eduardo B. Google Scholar
Duprat, Joao P. Autor UNIFESP Google Scholar
Freymueller, Edna Autor UNIFESP Google Scholar
Institution Hosp Reg San Jose
Universidade Federal de São Paulo (UNIFESP)
Ctr Paradis Monticelli
Abstract Purpose: the aim of this study was to determine the effects of subretinal injection of indocyanine green (ICG), infracyanine (IfCG), and balanced salt solution (BSS) in rabbits.Methods: Ten (10) animals were subjected to a subretinal injection of 0.05% ICG (279 mOsm), 0.5% IfCG (276 mOsm), and BSS (300 mOsm) used as a control. Animals were examined at 6, 12, and 24 h and 14 days following the surgical procedure by indirect binocular ophthalmoscopy, fluorescein angiography (FA), and light and transmission electron microscopy.Results: the subretinal injection of ICG caused damage to all retinal layers and retinal pigment epithelium (RPE) during the entire follow-up. Subretinal injection of IfCG resulted in abnormalities of the photoreceptor outer segments (POSs) during the entire follow-up; however, abnormalities of the photoreceptor inner segments (PISs) and outer nuclear layer (ONL) were observed only 24 h and 14 days after surgery; no RPE damage was observed. FA showed that window defects were more prominent in the subretinal ICG bleb position than the IfCG-related area. BSS caused only abnormalities of the POS layer and no RPE alterations.Conclusions: Subretinal injection of 0.05% ICG results in more significant retinal damage than 0.5% IfCG. in this model, iodine-free IfCG demonstrates a safer profile than a tenfold lower concentration of ICG, which contains iodine in its composition.
Language English
Date 2008-02-01
Published in Journal of Ocular Pharmacology and Therapeutics. New Rochelle: Mary Ann Liebert Inc, v. 24, n. 1, p. 52-61, 2008.
ISSN 1080-7683 (Sherpa/Romeo, impact factor)
Publisher Mary Ann Liebert Inc
Extent 52-61
Access rights Closed access
Type Article
Web of Science ID WOS:000252814200007

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