Ultrastructural evaluation of gingival glycosaminoglycans in ovariectomized rats: Effects of steroid hormone therapy

Ultrastructural evaluation of gingival glycosaminoglycans in ovariectomized rats: Effects of steroid hormone therapy

Author Calio, Pedro Luiz Autor UNIFESP Google Scholar
Simoes, Ricardo Santos Autor UNIFESP Google Scholar
Oliveira-Filho, Ricardo Martins Google Scholar
Maciel, Gustavo Arantes Rosa Google Scholar
Simoes, Manuel de Jesus Autor UNIFESP Google Scholar
Baracat, Edmund C. Autor UNIFESP Google Scholar
Soares, Jose Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Background/Aims: To evaluate the behavior of glycosaminoglycans (GAGs) in rat gingiva and the effects of lack of sexual steroids and the hormonal therapy with estrogen and dexamethasone (DEX). Methods: 40 female rats were divided into four groups: GI: animals in permanent estrus; GII: ovariectomized (OVX) animals + vehicle; GIII: OVX animals treated with 17 beta-estradiol benzoate (10 mu g/kg), and GIV: OVX animals treated with 17 beta-estradiol benzoate (10 mu g/kg) + DEX (3 mg/kg). After treatment, the gingiva was removed and its GAGs content was evaluated by electronic microscopy after stained by cuprolinic blue technique. Results: the electron-microscopic data showed that low values of chondroitin sulfate were found in castrated animals (35.05 +/- 3.58%) compared to other groups (GI: 41.17 +/- 1.13; GIII: 48.04 +/- 2.60; GIV: 49.09 +/- 2.68%). in contrast, the amount of dermatan sulfate in GII (57.70 +/- 2.50%) was higher than in the other groups (GI: 46.12 +/- 1.30; GIII: 42.65 +/- 2.98; GIV: 42.68 +/- 5.43%). Conclusions: GAGs may be influenced by estradiol, and DEX did not seem to antagonize the role of estradiol in the GAGs of gingiva. the histotypical structure of gingiva is related to the amount of chondroitin sulfate. Consequently, the estrogen therapy may be important for gingival health. Copyright (C) 2007 S. Karger AG, Basel.
Keywords estrogen therapy
gingival glycosaminoglycans
glycosaminoglycans
hormonal therapy, dexamethasone
hormonal therapy, estrogen
steroid hormone therapy
Language English
Date 2008-01-01
Published in Gynecologic and Obstetric Investigation. Basel: Karger, v. 65, n. 1, p. 12-17, 2008.
ISSN 0378-7346 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 12-17
Origin http://dx.doi.org/10.1159/000106499
Access rights Closed access
Type Article
Web of Science ID WOS:000251661400003
URI http://repositorio.unifesp.br/handle/11600/30349

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