Enhancement of europium emission band of europium tetracycline complex in the presence of cholesterol

Enhancement of europium emission band of europium tetracycline complex in the presence of cholesterol

Author Silva, Flávia Rodrigues de Oliveira Google Scholar
Samad, Ricardo Elgul Google Scholar
Gomes, Laercio Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
IPEN CNEN SP
Abstract We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H2O2, the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in a simple method to measure the cholesterol quantity in a solution. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems.
Keywords cholesterol
europium
fluorescence
optical spectroscopy
Language English
Date 2008-01-01
Published in Journal of Fluorescence. New York: Springer/plenum Publishers, v. 18, n. 1, p. 169-174, 2008.
ISSN 1053-0509 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 169-174
Origin http://dx.doi.org/10.1007/s10895-007-0260-7
Access rights Closed access
Type Article
Web of Science ID WOS:000252296800020
URI http://repositorio.unifesp.br/handle/11600/30268

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