Dimethyl sulfoxide as a block to the deleterious effect of nicotine in a random skin flap in the rat

Dimethyl sulfoxide as a block to the deleterious effect of nicotine in a random skin flap in the rat

Author Leite, Mauro Toledo Autor UNIFESP Google Scholar
Gomes, Heitor Carvalho Autor UNIFESP Google Scholar
Percario, Sandro Autor UNIFESP Google Scholar
Abrantes, Claudia Regina Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background: in plastic surgery, a causal relationship between heavy smoking and flap necrosis has been shown. the deleterious effect of nicotine in random skin flaps in rats has also been proven, being related to vasoconstriction and possibly reactive oxygen species. This study aimed to verify the capacity of dimethyl sulfoxide, an antioxidant, to block the deleterious effect of nicotine in a random skin flap.Methods: Fourteen adult male Wistar-EPM rats were distributed at random into two groups of seven animals. the nicotine group received this drug subcutaneously (1.2 mg/kg/day), for I week before flap elevation. the nicotine plus dimethyl sulfoxide group followed the same routine. Five minutes before the surgical procedure, rats in the nicotine group received distilled water orally and rats in the nicotine plus dimethyl sulfoxide group received dimethyl sulfoxide orally (2 ml/kg). Blood and skin tissue samples were collected to allow determination of malondialdehyde levels.Results: the nicotine group had a mean value of 40.2 percent and the nicotine plus dimethyl sulfoxide group had a mean value of 20.6 percent necrosis (p = 0.009). Malondialdehyde levels in both serum and skin samples were lower in the animals that received dimethyl sulfoxide.Conclusion: the deleterious effect of nicotine was effectively blocked by dimethyl sulfoxide.
Language English
Date 2007-12-01
Published in Plastic and Reconstructive Surgery. Philadelphia: Lippincott Williams & Wilkins, v. 120, n. 7, p. 1819-1822, 2007.
ISSN 0032-1052 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 1819-1822
Origin http://dx.doi.org/10.1097/01.prs.0000287134.60766.23
Access rights Closed access
Type Article
Web of Science ID WOS:000251668400008
URI http://repositorio.unifesp.br/handle/11600/30198

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