Exposure to arsenic at levels found in US drinking water modifies expression in the mouse lung

Exposure to arsenic at levels found in US drinking water modifies expression in the mouse lung

Author Andrew, Angeline S. Google Scholar
Bernardo, Viviane Autor UNIFESP Google Scholar
Warnke, Linda A. Google Scholar
Davey, Jennifer C. Google Scholar
Hampton, Thomas Google Scholar
Mason, Rebecca A. Google Scholar
Thorpe, Jessica E. Google Scholar
Ihnat, Michael A. Google Scholar
Hamilton, Joshua W. Google Scholar
Institution Dartmouth Med Sch
Dartmouth Coll
Universidade Federal de São Paulo (UNIFESP)
Univ Oklahoma
Abstract The mechanisms of action of drinking water arsenic in the lung and the threshold for biologic effects remain controversial. Our study utilizes Affymetrix 22,690 transcript oligonucleotide microarrays to assess the long-term effects of increasing doses of drinking water arsenic on expression levels in the mouse lung. Mice were exposed at levels commonly found in contaminated drinking water wells in the United States (0, 0.1, 1 ppb), as well as the 50 ppb former maximum contaminant level, for 5 weeks. the expression profiles revealed modification of a number of important signaling pathways, many with corroborating evidence of arsenic responsiveness. We observed statistically significant expression changes for transcripts involved in angiogenesis, lipid metabolism, oxygen transport, apoptosis, cell cycle, and immune response. Validation by reverse transcription-PCR and immunoblot assays confirmed expression changes for a subset of transcripts. These data identify arsenic-modified signaling pathways that will help guide investigations into mechanisms of arsenic's health effects and clarify the threshold for biologic effects and potential disease risk.
Keywords arsenic
apoptosis
cell cycle
drinking water
immune response
lung
microarray
oxygen transport
Language English
Date 2007-11-01
Published in Toxicological Sciences. Oxford: Oxford Univ Press, v. 100, n. 1, p. 75-87, 2007.
ISSN 1096-6080 (Sherpa/Romeo, impact factor)
Publisher Oxford Univ Press
Extent 75-87
Origin http://dx.doi.org/10.1093/toxsci/kfm200
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000250686600011
URI http://repositorio.unifesp.br/handle/11600/30106

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