Effect of the antimicrobial peptide gomesin against different life stages of Plasmodium spp.

Effect of the antimicrobial peptide gomesin against different life stages of Plasmodium spp.

Author Moreira, Cristina K. Google Scholar
Rodrigues, Flavia G. Google Scholar
Ghosh, Anil Google Scholar
Varotti, Fernando de P. Google Scholar
Miranda, Antonio Google Scholar
Daffre, Sirlei Google Scholar
Jacobs-Lorena, Marcelo Google Scholar
Moreira, Luciano A. Google Scholar
Institution Fundacao Oswaldo Cruz
Johns Hopkins Bloomberg Sch Publ Hlth
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract While seeking strategies for interfering with Plasmodium development in vertebrate/invertebrate hosts, we tested the activity of gomesin, an antimicrobial peptide isolated from the hemocytes of the spider Acanthoscurria gomesiana. Gomesin was tested against asexual, sexual and pre-sporogonic forms of Plasmodium falciparum and Plasmodium berghei parasites. the peptide inhibited the in vitro growth of intraerythrocytic forms of P. falciparum. When gomesin was added to in vitro, culture of P. berghei mature gametocytes, it significantly inhibited the exflagellation of male gametes and the formation of ookinetes. in vivo, the peptide reduced the number of oocysts of both Plasmodium species in Anopheles stephensi mosquitoes, and did not appear to affect the mosquitoes. These properties make gomesin an excellent candidate as a transmission blocking agent for the genetic engineering of mosquitoes. (c) 2007 Elsevier Inc. All rights reserved.
Keywords antimicrobial peptide
gomesin
malaria
mosquito
ookinete
oocyst
exflagellation
Language English
Date 2007-08-01
Published in Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 116, n. 4, p. 346-353, 2007.
ISSN 0014-4894 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 346-353
Origin http://dx.doi.org/10.1016/j.exppara.2007.01.022
Access rights Closed access
Type Article
Web of Science ID WOS:000247756400003
URI http://repositorio.unifesp.br/handle/11600/29906

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