Association of endothelial nitric oxide synthase gene intron 4 polymorphism with end-stage renal disease

Association of endothelial nitric oxide synthase gene intron 4 polymorphism with end-stage renal disease

Author Bellini, Maria H. Google Scholar
Figueira, Marcia N. Google Scholar
Piccoli, Michelly F. Google Scholar
Marumo, Julio T. Google Scholar
Cendoroglo, Maysa S. Google Scholar
Neto, Miguel C. Google Scholar
Dalboni, Maria Aparecida Autor UNIFESP Google Scholar
Batista, Marcelo C. Google Scholar
Goes, Miguel A. Google Scholar
Schor, Nestor Google Scholar
Institution Cidade Univ
Universidade Federal de São Paulo (UNIFESP)
Abstract Background: Nitric oxide (NO) released from endothelial cells is related to the maintenance of physiological vascular tone. the impairment of endothelial NO generation brought about by gene polymorphism is considered one of the deterioration factors in progressive renal disease. in the endothelial nitric oxide synthase (eNOS) intron 4 polymorphism, the presence of the aa genotype has been associated with cardiovascular and renal disease. the aim of this study was to investigate the presence of eNOS gene intron 4 polymorphism in patients with end-stage renal disease (ESRD).Methods: A total of 114 patients and 94 controls were studied. DNA specimens were extracted from blood and amplified by polymerase chain reaction. the alleles were separated by agarose gel electrophoresis. Genotype distribution and allele frequencies were compared between groups using the chi-squared test.Results: Statistical analysis revealed that the frequency of the eNOS4 genotype aa was significantly different in ESRD patients and in controls (P = 0.016, OR = 2.07, CI 95%: 1.14-3.74). There was also a statistically significant difference between ESRD patients and controls regarding allele carriers (P = 0.004; OR = 2.26; CI 95%: 1.29-3.96). When the frequencies of allele carriers in the diabetic nephropathy group and in the control group were compared, a significant difference was found (P = 0.034, OR = 2.28; CI 95%: 1.04-5.00).Conclusion: This study showed a strong correlation between eNOS4a polymorphism and end-stage renal disease.
Keywords 27-bp repeats in intron 4
chronic renal failure
diabetes mellitus
endothelial nitric oxide synthase
end-stage renal disease
gene polymorphism
Language English
Date 2007-06-01
Published in Nephrology. Malden: Wiley-Blackwell, v. 12, n. 3, p. 289-293, 2007.
ISSN 1320-5358 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 289-293
Origin http://dx.doi.org/10.1111/j.1440-1797.2007.00787.x
Access rights Closed access
Type Article
Web of Science ID WOS:000246888700012
URI http://repositorio.unifesp.br/handle/11600/29813

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